Description
| Product Name: | Mouse GPT2 Recombinant Protein |
| Product Code: | RPPB1741 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | GPT2 |
| Synonyms: | ALT2, AAT2, Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GPT2 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 7.5), 20% glycerol, and 2mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MQRAAVLVRR GSCPRASGPW GRSHSSAAAE ASAALKVRPE RSPRDRILTL ESMNPQVKAV EYAVRGPIVL KAGEIEMELQ RGIKKPFTEV IRANIGDAHA MGQQPITFLR QVMALCTYPN LLNSPSFPED AKKRARRILQ ACGGNSLGSY SASQGVNCIR EDVAAFITRR DGVPADPDNI YLTTGASDGI STILKLLVSG GGKSRTGVMI PIPQYPLYSA VISELDAVQV NYYLDEENCW ALNVDELRRA LRQAKDHCDP KVLCIINPGN PTGQVQSRKC IEDVIHFAWE EKLFLLADEV YQDNVYSPDC RFHSFKKVLY QMGHEYSSNV ELASFHSTSK GYMGECGYRG GYMEVINLHP EIKGQLVKLL SVRLCPPVSG QAAMDIVVNP PEPGEESFEQ FSREKEFVLG NLAKKAKLTE DLFNQVPGIQ CNPLQGAMYA FPRILIPAKA VEAAQSHKMA PDMFYCMKLL EETGICVVPG SGFGQREGTY HFRMTILPPV DKLKTVLHKV KDFHLKFLEQ |
| Biological Activity: | Greater than 50units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH7.5 at 37?. |
Glutamic-Pyruvate Transaminase 2 (GPT2) catalyzes the reversible transamination among alanine and 2-oxoglutarate to create pyruvate and glutamate. GPT2 expressed mainly in muscle, fat and kidney and participates in the intermediary metabolism of glucose and amino acids.
GPT2 Mouse Recombinant produced in E. coli is a single polypeptide chain containing 522 amino acids (1-522) and having a molecular mass of 60.1 kDa. Mouse GPT2 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GPT2: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.6.1.2; Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase; Mitochondrial Chromosomal Location of Human Ortholog: 16q12.1 Cellular Component: mitochondrial matrix Molecular Function:alanine transaminase activity; pyridoxal phosphate binding Biological Process: 2-oxoglutarate metabolic process; amino acid biosynthetic process; L-alanine catabolic process; L-alanine metabolic process Disease: Mental Retardation, Autosomal Recessive 49 |
| NCBI Summary: | This gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in this gene has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] |
| UniProt Code: | Q8TD30 |
| NCBI GenInfo Identifier: | 74730602 |
| NCBI Gene ID: | 84706 |
| NCBI Accession: | Q8TD30.1 |
| UniProt Secondary Accession: | Q8TD30,Q8N9E2, |
| UniProt Related Accession: | Q8TD30 |
| Molecular Weight: | 46,983 Da |
| NCBI Full Name: | Alanine aminotransferase 2 |
| NCBI Synonym Full Names: | glutamic pyruvate transaminase (alanine aminotransferase) 2 |
| NCBI Official Symbol: | GPT2�� |
| NCBI Official Synonym Symbols: | ALT2; GPT 2; MRT49�� |
| NCBI Protein Information: | alanine aminotransferase 2 |
| UniProt Protein Name: | Alanine aminotransferase 2 |
| UniProt Synonym Protein Names: | Glutamate pyruvate transaminase 2; GPT 2; Glutamic--alanine transaminase 2; Glutamic--pyruvic transaminase 2 |
| Protein Family: | Xanthine phosphoribosyltransferase |
| UniProt Gene Name: | GPT2�� |
| UniProt Entry Name: | ALAT2_HUMAN |
