Enzymes Recombinant Proteins

Mouse GPT Recombinant Protein (RPPB1737)

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SKU:
RPPB1737
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
Q8QZR5
Research Area:
Enzymes

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Mouse GPT Recombinant Protein
Product Code:RPPB1737
Size:10µg
Species:Mouse
Target:GPT
Synonyms:Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, GPT 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:GPT protein solution (0.5mg/ml) containing Phosphate Buffered Saline (pH7.4), 1mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMASQRND RIQASRNGLK GKVLTLDTMN PCVRRVEYAV RGPIVQRALE LEQELRQGVK KPFTEVIRAN IGDAQAMGQR PITFFRQVLA LCVYPNLLSS PDFPEDAKRR AERILQACGG HSLGAYSISS GIQPIREDVA QYIERRDGGI PADPNNIFLS�TGASDAIVTM LKLLVAGEGR ARTGVLIPIP QYPLYSAALA ELDAVQVDYY LDEERAWALD IAELRRALCQ ARDRCCPRVL CVINPGNPTG QVQTRECIEA VIRFAFEEGL FLMADEVYQD NVYAEGSQFH SFKKVLTEMG PPYATQQELA SFHSVSKGYM GECGFRGGYV EVVNMDAEVQ�KQMAKLMSVR LCPPVPGQAL MGMVVSPPTP SEPSFKQFQA ERQEVLAELA AKAKLTEQVF NEAPGIRCNP VQGAMYSFPQ IQLPLKAVQR AQDLGLAPDM FFCLCLLEET GICVVPGSGF GQQEGTYHFR MTILPPMEKL RVLLEKLRHF HAKFTHEYS
Biological Activity:Specific activity is > 40units/mg, and is defined as the amount of enzyme that convert 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C.�

GPT catalyzes the reversible transamination between alanine and 2-oxoglutarate to create pyruvate and glutamate. GPT has a crucial part in the intermediary metabolism of glucose and amino acids. GPT is broadly used as an indicator of liver reliability or hepatocellular destruction in clinical tests.

GPT Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 519 amino acids (1-496 a.a) and having a molecular mass of 57.5kDa.GPT is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
UniProt Code:Q8QZR5
NCBI GenInfo Identifier:46576612
NCBI Gene ID:76282
NCBI Accession:Q8QZR5.3
UniProt Related Accession:Q8QZR5
Molecular Weight:Predicted Molecular Mass: 58.7kDaAccurate Molecular Mass: 59kDa
NCBI Full Name:Alanine aminotransferase 1
NCBI Synonym Full Names:glutamic pyruvic transaminase, soluble
NCBI Official Symbol:Gpt��
NCBI Official Synonym Symbols:ALT; ALT1; Gpt1; Gpt-1; 1300007J06Rik; 2310022B03Rik��
NCBI Protein Information:alanine aminotransferase 1
UniProt Protein Name:Alanine aminotransferase 1
UniProt Synonym Protein Names:Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1
Protein Family:Alanine aminotransferase
UniProt Gene Name:Gpt��
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