Enzymes Recombinant Proteins

Mouse GOT1 Recombinant Protein (RPPB1724)

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RPPB1724

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Mouse GOT1 Recombinant Protein
Product Code:RPPB1724
Size:20µg
Species:Mouse
Target:GOT1
Synonyms:Aspartate aminotransferase, cytoplasmic, cAspAT, Cysteineaminotransferase, cytoplasmic, Cysteine transaminase, cytoplasmic, cCAT,Glutamate oxaloacetate transaminase 1, Transaminase A.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:GOT1 protein solution (0.5mg/ml) containing Phosphatebuffered saline (pH7.4), 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greaterthan 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAPPSVF AQVPQAPPVL VFKLTADFRD DPDPRKVNLG VGAYRTDESQPWVLPVVRKV EQKIANDNSL NHEYLPILGL AEFRSCASRL VLGDNSPAIR ENRVGGVQSL GGTGALRIGADFLGRWYNGT DNKNTPIYVS SPTWENHNAV FSAAGFKDIR PYCYWDAEKR GLDLQGFLND LENAPEFSIF VLHACAHNPT GTDPTPEQWKQIAAVMQRRF LFPFFDSAYQ GFASGDLEKD AWAIRYFVSE GFELFCAQSF SKNFGLYNER VGNLTVVGKESDSVLRVLSQ MEKIVRITWS NPPAQGARIV AATLSDPELF KEWKGNVKTM ADRILTMRSE LRARLEALKT PGTWSHITEQIGMFSFTGLN PKQVEYLVNE KHIYLLPSGR INMCGLTTKN LDYVATSIHE AVTKIQ

GOT1 is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, which participate in amino acid metabolism and the urea and tricarboxylic acid cycles. Both enzymes are homodimeric and show close homology.

GOT1 Mouse Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 436 amino acids (1-413a.a) andhaving a molecular mass of 48.6kDa.GOT1 is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.

UniProt Protein Function:Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
UniProt Code:P05201
NCBI GenInfo Identifier:160298209
NCBI Gene ID:14718
NCBI Accession:NP_034454.2
UniProt Secondary Accession:P05201,Q3UJH8,
UniProt Related Accession:P05201
Molecular Weight:46,248 Da
NCBI Full Name:aspartate aminotransferase, cytoplasmic
NCBI Synonym Full Names:glutamic-oxaloacetic transaminase 1, soluble
NCBI Official Symbol:Got1��
NCBI Official Synonym Symbols:cCAT; Got-1; cAspAT; AI789014��
NCBI Protein Information:aspartate aminotransferase, cytoplasmic
UniProt Protein Name:Aspartate aminotransferase, cytoplasmic
UniProt Synonym Protein Names:Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A
Protein Family:Allatostatin
UniProt Gene Name:Got1��
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Additional Information

Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P05201
Research Area:
Enzymes
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Metabolism Recombinant Proteins

system_update_altDatasheetHuman GOT1 Recombinant Protein Glutamate Oxaloacetate Transaminase 1 (GOT1) is a cytoplasmic protein. GOT1 belongs to the class-I pyridoxal-phosphate-dependent aminotran