Mouse Metabolism ELISA Kits

Mouse Glutamate dehydrogenase / GDH ELISA Kit

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SKU:
MOFI00853
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8CFX1
Sensitivity:
0.094ng/ml
Range:
0.156-10ng/ml
ELISA Type:
Sandwich
Synonyms:
GDH, GLDH
Reactivity:
Mouse
Research Area:
Metabolism

Description

Mouse Glutamate dehydrogenase / GDH ELISA Kit

GDH is an enzyme found in various organisms, including mice. It plays a crucial role in the metabolism of the amino acid glutamate. The Mouse GDH ELISA Kit is specifically designed for the quantitative detection and measurement of Glutamate Dehydrogenase levels in mouse samples. This kit enables the investigation of GLUD1's role in cellular metabolism, energy production, and related research areas.

system_update_alt Datasheet system_update_alt MSDS

Key Features

Save Time Pre-coated 96 well plate
Quick Start Kit includes all necessary reagents
Publication Ready Reproducible and reliable results

Overview

Product Name:

Mouse Glutamate dehydrogenase / GDH ELISA Kit

Product Code:

MOFI00853

Size:

96 Assays

Alias:

GDH, GLDH

Detection Method:

Sandwich ELISA

Application:

This immunoassay kit allows for the in vitro quantitative determination of Mouse GLDH concentrations in serum plasma and other biological fluids.

Sensitivity:

0.094ng/ml

Range:

0.156-10ng/ml

Storage:

4°C for 6 months

Note:

For Research Use Only

Additional Information

Recovery

Matrices listed below were spiked with certain level of Mouse GDH and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse GDH in samples.

Matrix

Recovery Range (%)

Average (%)

serum (n=5)

95-103

98

EDTA plasma (n=5)

86-104

94

UFH plasma (n=5)

86-100

92

Linearity:

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse GDH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

Serum (n=5)

86-103%

91-104%

86-102%

EDTA plasma (n=5)

90-98%

84-99%

86-101%

UFH Plasma (n=5)

86-97%

83-100%

83-99%

Intra Assay

CV < 8%

Inter Assay

CV < 10%

Kit Components

Component Quantity Storage

ELISA Microplate (Dismountable)

8x12 strips

4°C for 6 months

Lyophilized Standard

2

4°C/ -20°C

Sample/Standard Dlution Buffer

20ml

4°C

Biotin-labeled Antibody (Concentrated)

120ul

4°C (Protection from light)

Antibody Dilution Buffer

10ml

4°C

HRP-Streptavidin Conjugate (SABC)

120ul

4°C (Protect from light)

SABC Dilution Buffer

10ml

4°C

TMB Substrate

10ml

4°C (Protection from light)

Stop Solution

10ml

4°C

Wash Buffer (25X)

30ml

4°C

Plate Sealer

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