Cytokines Recombinant Proteins

Mouse GDF5 Recombinant Protein (RPPB0305)

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SKU:
RPPB0305
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P43027
Research Area:
Cytokines

Description

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Product Name:Mouse GDF5 Recombinant Protein
Product Code:RPPB0305
Size:50µg
Species:Mouse
Target:GDF5
Synonyms:Bmp-14, Bp, GDF-5, Bone morphogenetic protein 14, GDF5, Growth/differentiation factor 5.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:GDF-5 protein was lyophilized from a 0.2�m filtered concentrated solution in 30% Acetonitrile and 0.1% TFA.
Solubility:It is recommended to reconstitute the lyophilized GDF5 in sterile 4mM HCl not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized GDF5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDF-5 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity:Greater than 96.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:APLANRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR
Biological Activity:The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0�g/ml, corresponding to a specific activity of > 1000IU/mg.

GDF-5 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development.

GDF5 Mouse Recombinant produced in E.coli is a non-glycosylated disulfide linked homodimer containing 2 chains of 120 amino acids and having a molecular mass of 27.2kDa.The GDF-5 is purified by proprietary chromatographic techniques.

UniProt Protein Function:GDF5: a cytokine that is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. These cytokines are characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Binds to bone morphogenetic protein receptors (BMPRs), a family of transmembrane serine/threonine kinases. BMPRs are involved in bone and cartilage formation. Chondrogenic signaling is mediated by the high-affinity receptor BMPR1B. Defects in GDF5 are the cause of acromesomelic chondrodysplasia Grebe type (AMDG), acromesomelic chondrodysplasia Hunter-Thompson type (AMDH), brachydactyly type C (BDC), Du Pan syndrome (DPS), symphalangism proximal syndrome (SYM1), multiple synostoses syndrome type 2 (SYNS2), and brachydactyly type A2 (BDA2). Genetic variations in GDF5 are associated with susceptibility to osteoarthritis type 5 (OS5). Belongs to the TGF-beta family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide

Cellular Component: extracellular space; membrane; extracellular region; plasma membrane

Molecular Function:identical protein binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding; receptor binding

Biological Process: hindlimb morphogenesis; regulation of multicellular organism growth; BMP signaling pathway; forelimb morphogenesis; positive regulation of chondrocyte differentiation; cartilage development; regulation of MAPKKK cascade; transmembrane receptor protein serine/threonine kinase signaling pathway; chondrocyte differentiation; negative regulation of neuron apoptosis; positive regulation of neuron differentiation; cell development; embryonic limb morphogenesis; negative regulation of epithelial cell proliferation; growth

UniProt Code:P43027
NCBI GenInfo Identifier:341940738
NCBI Gene ID:14563
NCBI Accession:P43027.2
UniProt Secondary Accession:P43027,A2ARK2,
UniProt Related Accession:P43027
Molecular Weight:54,895 Da
NCBI Full Name:Growth/differentiation factor 5
NCBI Synonym Full Names:growth differentiation factor 5
NCBI Official Symbol:Gdf5��
NCBI Official Synonym Symbols:bp; brp; BMP-14; Cdmp-1��
NCBI Protein Information:growth/differentiation factor 5; GDF-5; brachypodism; bone morphogenetic protein 14; cartilage-derived morphogenetic protein-1
UniProt Protein Name:Growth/differentiation factor 5
UniProt Synonym Protein Names:Bone morphogenetic protein 14; BMP-14
UniProt Gene Name:Gdf5��
UniProt Entry Name:GDF5_MOUSE
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