Enzymes Recombinant Proteins

Mouse GAPDH Recombinant Protein (RPPB1674)

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SKU:
RPPB1674
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P16858
Research Area:
Enzymes

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Mouse GAPDH Recombinant Protein
Product Code:RPPB1674
Size:10µg
Species:Mouse
Target:GAPDH
Synonyms:Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, Gapdh, Gapd, Glyceraldehyde-3-phosphate dehydrogenase isoform 2, G3PD, GAPD, HEL-S-162eP.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:GAPDH protein solution (0.25mg/ml) containing Phosphate buffered saline (pH7.4), 20% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMVKVGVN GFGRIGRLVT RAAICSGKVE IVAINDPFID LNYMVYMFQY DSTHGKFNGT VKAENGKLVI NGKPITIFQE RDPTNIKWGE AGAEYVVEST GVFTTMEKAG AHLKGGAKRV IISAPSADAP MFVMGVNHEK YDNSLKIVSN ASCTTNCLAP LAKVIHDNFG IVEGLMTTVH AITATQKTVD GPSGKLWRDG RGAAQNIIPA STGAAKAVGK VIPELNGKLT GMAFRVPTPN VSVVDLTCRL EKPAKYDDIK KVVKQASEGP LKGILGYTED QVVSCDFNSN SHSSTFDAGA GIALNDNFVK LISWYDNEYG YSNRVVDLMA YMASKE
Biological Activity:Specificactivity is > 40 units/mg, and is defined as the amount of enzyme thatconvert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate perminute at pH 8.5 at 37C.

GAPDH is a catalytic enzyme normally known to play a role in glycolysis. GAPDH exists as a tetramer composed of 36-kDa subunits and has a range of intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, GAPDH has function in intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. GAPDH catalyzes a vital energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

GAPDH Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 356 amino acids (1-333a.a.) and having a molecular mass of 38.2kDa.GAPDH is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:GAPDH: a multifunctional enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. A key glycolytic enzyme that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. An important enzyme for energy metabolism, and the production of ATP and pyruvate through anaerobic glycolysis in the cytoplasm. Additionally, it participates in apoptosis, membrane trafficking, iron metabolism, nuclear activities and receptor mediated cell signaling. Its subcellular localization changes reflecting its multiple activities. Is cytosolic, but is also localized in the membrane, the nucleus, polysomes, the ER and the Golgi. Participates in transcription, RNA transport, DNA replication and apoptosis. S-nitrosylation on Cys-152 following apoptotic stimulates its interaction with SIAH2, which in turn moderates its translocation into the nucleus. Mediates cysteine S-nitrosylation of nuclear target proteins including SIRT1, HDAC2 and DNA-PK. Deregulated in lung cancer, renal cancer, breast cancer, gastric cancer, glioma, liver cancer, colorectal cancer, melanoma, prostatic cancer, pancreatic cancer and bladder cancer. Its increased expression and enzymatic activity is associated with cell proliferation and tumorigenesis, Oxidative stress impairs GAPDH catalytic activity and leads to cellular aging and apoptosis. In experimental animal models, injection of GAPDH antagonists induces apoptosis and blocks Hep3B tumor progression, suggesting a therapeutic potential of targeting GAPDH in human hepatocellular carcinoma
UniProt Protein Details:

Protein type:EC 1.2.1.12; Oxidoreductase; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; lipid particle; membrane; microtubule cytoskeleton; mitochondrion; nuclear membrane; nucleus; plasma membrane; ribonucleoprotein complex; vesicle

Molecular Function:glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; identical protein binding; microtubule binding

Biological Process: apoptosis; carbohydrate metabolic process; gluconeogenesis; glycolysis; microtubule cytoskeleton organization and biogenesis; multicellular organismal development; negative regulation of translation; neuron apoptosis; protein stabilization

UniProt Code:P16858
NCBI GenInfo Identifier:120702
NCBI Gene ID:100042025
NCBI Accession:P16858.2
UniProt Secondary Accession:P16858,Q0QEU0, Q3THM2, Q3TUI2, Q3UMT2, Q4V783, Q569X2 Q569X5, Q5U410, A6H6A8,
Molecular Weight:35,810 Da
NCBI Full Name:Glyceraldehyde-3-phosphate dehydrogenase
NCBI Synonym Full Names:glyceraldehyde-3-phosphate dehydrogenase, pseudogene 15
NCBI Official Symbol:Gapdh-ps15��
NCBI Official Synonym Symbols:Gm20899��
NCBI Protein Information:glyceraldehyde-3-phosphate dehydrogenase, pseudogene 15
UniProt Protein Name:Glyceraldehyde-3-phosphate dehydrogenase
UniProt Synonym Protein Names:Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)
UniProt Gene Name:Gapdh��
UniProt Entry Name:G3P_MOUSE
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system_update_altDatasheetHuman GAPDH Recombinant Protein Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved i