Growth Factors & Cytokines Recombinant Proteins

Mouse FGF 8 Recombinant Protein (RPPB0219)

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SKU:
RPPB0219
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P37237
Research Area:
Growth Factors & Cytokines

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Mouse FGF 8 Recombinant Protein
Product Code:RPPB0219
Size:25µg
Species:Mouse
Target:FGF 8
Synonyms:Fibroblast growth factor 8, FGF-8, Androgen-induced growth factor, AIGF, Heparin-binding growth factor 8, HBGF-8, Fgf8.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:FGF-8 protein was lyophilized from a 0.2�m filtered concentrated solution in PBS, pH 7.4.
Solubility:It is recommended to reconstitute the lyophilized FGF-8 in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized FGF-8 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-8 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by: (a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:QVRSAAQKRG PGAGNPADTL GQGHEDRPFG QRSRAGKNFT NPAPNYPEEG SKEQRDSVLP KVTQRHVREQ SLVTDQLSRR LIRTYQLYSR TSGKHVQVLA NKRINAMAED GDPFAKLIVE TDTFGSRVRV RGAETGLYIC MNKKGKLIAK SNGKGKDCVF TEIVLENNYT ALQNAKYEGW YMAFTRKGRP RKGSKTRQHQ REVHFMKRLP RGHHTTEQSL RFEFLNYPPF TRSLRGSQRT WAPEPR
Biological Activity:The ED50, as determined by the dose-dependent a cell proliferation assay using NR6R-3T3 mouse fibroblast cells is <25 ng/ml in the presence of 0.1 ug/ml heprin, corresponding to a specific activity of > 4.0�104 units/mg.

FGF8 is part of the fibroblast growth factor family. FGF family members have wide mitogenic and cell survival activities, and participate in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF8 supports androgen and anchorage independent growth of mammary tumor cells. FGF8 over expression increases tumor growth and angiogensis. The adult expression of FGF-8 gene is restricted to testes and ovaries. FGF8 functions as an embryonic epithelial factor. FGF8 takes part in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination.

FGF-8 Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 246 amino acids and having a molecular mass of 28.1kDa.The FGF-8 is purified by proprietary chromatographic techniques.

UniProt Protein Function:FGF8: Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6). Kallmann syndrome is a disorder that associates hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone- synthesizing neurons. In some patients other developmental anomalies can be present, which include renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. In some cases anosmia may be absent or inconspicuous. Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH). IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative splicing.Protein type: Secreted, signal peptide; Secreted; CytokineCellular Component: external side of plasma membrane; extracellular spaceMolecular Function: chemoattractant activity; growth factor activity; type 1 fibroblast growth factor receptor binding; type 2 fibroblast growth factor receptor bindingBiological Process: adrenocorticotropin hormone secreting cell differentiation; apoptosis; blood vessel remodeling; BMP signaling pathway; cell fate commitment; cell proliferation in forebrain; central nervous system neuron development; determination of left/right symmetry; dorsal/ventral axon guidance; embryonic development ending in birth or egg hatching; embryonic heart tube development; embryonic hindlimb morphogenesis; fibroblast growth factor receptor signaling pathway; forebrain dorsal/ventral pattern formation; forebrain morphogenesis; forebrain neuron development; generation of neurons; gonad development; heart development; heart looping; heart morphogenesis; induction of an organ; inner ear morphogenesis; kidney development; limb morphogenesis; lung development; male genitalia development; MAPKKK cascade; mesodermal cell migration; midbrain-hindbrain boundary development; motor axon guidance; negative regulation of apoptosis; negative regulation of cardiac muscle development; negative regulation of neuron apoptosis; neural plate morphogenesis; otic vesicle formation; pallium development; patterning of blood vessels; pharyngeal system development; positive regulation of cell proliferation; positive regulation of mitosis; positive regulation of organ growth; regulation of odontogenesis of dentine-containing teeth; response to oxidative stress; subpallium development; telencephalon development; thyroid gland development; thyroid stimulating hormone secreting cell differentiation; ureteric bud branching; Wnt receptor signaling pathway through beta-catenin
UniProt Protein Details:
NCBI Summary:
UniProt Code:P37237
NCBI GenInfo Identifier:585131
NCBI Gene ID:14179
NCBI Accession:P37237.1
UniProt Secondary Accession:P37237
UniProt Related Accession:P37237
Molecular Weight:23,522 Da
NCBI Full Name:Fibroblast growth factor 8
NCBI Synonym Full Names:fibroblast growth factor 8
NCBI Official Symbol:Fgf8��
NCBI Official Synonym Symbols:Aigf; Fgf-8��
NCBI Protein Information:fibroblast growth factor 8
UniProt Protein Name:Fibroblast growth factor 8
UniProt Synonym Protein Names:Androgen-induced growth factor; AIGF; Heparin-binding growth factor 8; HBGF-8
Protein Family:
UniProt Gene Name:Fgf8��
UniProt Entry Name:FGF8_MOUSE
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