Mouse F4/80 Monoclonal Antibody (PE/Cyanine5 Conjugated) [CI:A3-1](AGEL0570)

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SKU:
AGEL0570

Description

Antibody Name:Mouse F4/80 Monoclonal Antibody (PE/Cyanine5 Conjugated)
Antibody Code:AGEL0570
Size:50µg
Clone:CI:A3-1
Synonyms:Adhesion G protein-coupled receptor E1,Adgre1,Cell surface glycoprotein F4/80,EGF-like module receptor 1,Adgre1,Emr1, Gpf480
Host Species:Rat
Species Reactivity:Mouse
Applications:FC
Concentration:1mg/mL
Isotype:IgG2a,k
Conjugation:PE/Cyanine5
Applications:FC
Recommended Dilution:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10e6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Storage Buffer:PBS with 0.05% Proclin300, 1% BSA
Storage:Store at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Gene ID:13733
Uniprot:Q61549
Background:F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.
Mouse CD19 Monoclonal Antibody (FITC Conjugated)Mouse abdominal macrophages elicited by starch broth are stained with Anti-Mouse F4/80 Monoclonal Antibody(PE/Cyanine5 Conjugated)[Used at 0.02 ?g/106 cells dilution](filled gray curve). Unstained macrophages (blank black curve) are used as control.
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Additional Information

Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Applications:
FC
Reactivity:
Mouse
Host Species:
Rat
Isotype:
IgG2a,k
Clone:
CI:A3-1
Conjugation:
PE/Cy5
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