Enzymes Recombinant Proteins

Mouse EOGT Recombinant Protein (RPPB1624)

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SKU:
RPPB1624
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
Q8BYW9
Research Area:
Enzymes

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Mouse EOGT Recombinant Protein
Product Code:RPPB1624
Size:10µg
Species:Mouse
Target:EOGT
Synonyms:EGF domain-specific O-linked N-acetylglucosaminetransferase, Extracellular O-linked N-acetylglucosamine transferase.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered colorless solution.
Formulation:EOGTprotein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10%glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles.
Purity:Greaterthan 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:DKAHSEADDA PGKALYDYSS LRLPAEHIPF FLHNNRHVAS VCREDSHCPYKKHLENLNYC WGYEKSCAPE FRFGSPVCSY VDLGWTDTLE SAQDMFWRQA DFGYARERLG EIRTICQPERASDSSLVCSR YLQYCRATGL YLDLRNIKRN HDRFKEDFLQ GGEIGGYCKL DSHALVSEGQ RKSPLQSWFAELQGYTQLNF RPIEDAKCDI VVEKPTYFMK LDAGINMYHH FCDFLNLYLT QHVNNSFSTD VYIVMWDTSTYGYGDLFSDT WKAFTDYDVI HLKTYDSKKV CFKEAVFSLL PRMRYGLFYN TPLISGCQNT GLFRAFSQHVLHRLNITQEG PKDGKVRVTI LARSTEYRKI LNQDELVNAL KTVSTFEVRV VDYKYRELGF LDQLRITHNTDIFIGMHGAG LTHLLFLPDW AAVFELYNCE DERCYLDLAR LRGIHYITWR KPSKVFPQDK GHHPTLGEHPKFTNYSFDVE EFMYLVLQAA EHVLQHPQWP FKKKHDELLE HHHHHH
UniProt Code:Q8BYW9

EGFDomain-Specific O-Linked N-Acetylglucosamine Transferase (EOGT) takes part inthe regulation of Notch receptor. EOGT catalyzesthe transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine/ threonineresidue in extracellular proteins resulting in their modification with a beta-linkedN-acetylglucosamine (O-GlcNAc). EOGT mainlyglycosylates the Thr residue positioned between the fifth and sixth conservedcysteines of folded EGF-like domains.

EOGT produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 516 amino acids (20-527 a.a.) andhaving a molecular mass of 60.4kDa (Migrates at 50-70kDa on SDS-PAGE underreducing conditions).EOGT is expressed with an 8 amino acid His tag at C-Terminusand purified by proprietary chromatographic techniques.

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