Description
| Product Name: | Mouse ENO1 Recombinant Protein |
| Product Code: | RPPB1613 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | ENO1 |
| Synonyms: | 0610008I15, AL022784, Eno-1, MBP-1, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 1, Non-neural enolase, NNE, Eno1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | ENO1 protein solution (1mg/ml) containing 20mM Tris-Hcl buffer (pH8.5), 40% glycerol and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMSILRIH AREIFDSRGN PTVEVDLYTA KGLFRAAVPS GASTGIYEAL ELRDNDKTRF MGKGVSQAVE HINKTIAPAL VSKKVNVVEQ EKIDKLMIEM DGTENKSKFG ANAILGVSLA VCKAGAVEKG VPLYRHIADL AGNPEVILPV PAFNVINGGSHAGNKLAMQE FMILPVGASS FREAMRIGAE VYHNLKNVIK EKYGKDATNV GDEGGFAPNI LENKEALELL KTAIAKAGYT DQVVIGMDVA ASEFYRSGKY DLDFKSPDDP SRYITPDQLA DLYKSFVQNY PVVSIEDPFD QDDWGAWQKF TASAGIQVVG DDLTVTNPKR IAKAASEKSCNCLLLKVNQI GSVTESLQAC KLAQSNGWGV MVSHRSGETE DTFIADLVVG LCTGQIKTGA PCRSERLAKY NQILRIEEEL GSKAKFAGRS FRNPLAK |
| Biological Activity: | Specific activity is greater than 6,000 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0 pmole of 2-phosphoglycerate to phosphoenolpyruvate per minute at pH 6.5 at 37C in a couple system with PK and LDH. |
ENO1 is a homodimeric soluble protein that encodes a smaller monomeric structural lens protein, tau-crystallin. ENO1 is a glycolytic enzyme expressed in mainly all tissues. ENO1 isoenzyme full length protein is found in the cytoplasm. The shorter protein is formed from another translation start that is restricted to the nucleus, and binds to a component in the c-myc promoter. ENO1 is involved in anaerobic metabolism under hypoxic conditions and plays a role as a cell surface plasminogen receptor during tissue invasion. Irregular expression of Enolase-1 is linked with tumor progression in several cases of breast and lung cancer. Enolase-1 is as an auto antigen associated with Hashimoto's encephalopathy and severe asthma. ENO1 is the target protein of serum anti-endothelial antibody in Behcet's disease.
ENO1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 457 amino acids (1-434 a.a) and having a molecular mass of 24kDa.ENO1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses (). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production (). |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | P17182 |
| NCBI GenInfo Identifier: | 13637776 |
| NCBI Gene ID: | 13806 |
| NCBI Accession: | P17182.3 |
| UniProt Secondary Accession: | P17182,Q99KT7, Q9DCY7 |
| UniProt Related Accession: | P17182 |
| Molecular Weight: | 47,141 Da |
| NCBI Full Name: | Alpha-enolase |
| NCBI Synonym Full Names: | enolase 1, alpha non-neuron |
| NCBI Official Symbol: | Eno1�� |
| NCBI Official Synonym Symbols: | Eno-1; MBP-1; AL022784; 0610008I15�� |
| NCBI Protein Information: | alpha-enolase |
| UniProt Protein Name: | Alpha-enolase |
| UniProt Synonym Protein Names: | 2-phospho-D-glycerate hydro-lyase; Enolase 1; Non-neural enolase; NNE |
| Protein Family: | Alpha-enolase |
| UniProt Gene Name: | Eno1�� |
| UniProt Entry Name: |
