Enzymes Recombinant Proteins

Mouse ENO1 Recombinant Protein (RPPB1613)

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RPPB1613

Description

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Product Name:Mouse ENO1 Recombinant Protein
Product Code:RPPB1613
Size:10µg
Species:Mouse
Target:ENO1
Synonyms:0610008I15, AL022784, Eno-1, MBP-1, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 1, Non-neural enolase, NNE, Eno1.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:ENO1 protein solution (1mg/ml) containing 20mM Tris-Hcl buffer (pH8.5), 40% glycerol and 0.1M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMSILRIH AREIFDSRGN PTVEVDLYTA KGLFRAAVPS GASTGIYEAL ELRDNDKTRF MGKGVSQAVE HINKTIAPAL VSKKVNVVEQ EKIDKLMIEM DGTENKSKFG ANAILGVSLA VCKAGAVEKG VPLYRHIADL AGNPEVILPV PAFNVINGGSHAGNKLAMQE FMILPVGASS FREAMRIGAE VYHNLKNVIK EKYGKDATNV GDEGGFAPNI LENKEALELL KTAIAKAGYT DQVVIGMDVA ASEFYRSGKY DLDFKSPDDP SRYITPDQLA DLYKSFVQNY PVVSIEDPFD QDDWGAWQKF TASAGIQVVG DDLTVTNPKR IAKAASEKSCNCLLLKVNQI GSVTESLQAC KLAQSNGWGV MVSHRSGETE DTFIADLVVG LCTGQIKTGA PCRSERLAKY NQILRIEEEL GSKAKFAGRS FRNPLAK
Biological Activity:Specific activity is greater than 6,000 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0 pmole of 2-phosphoglycerate to phosphoenolpyruvate per minute at pH 6.5 at 37C in a couple system with PK and LDH.

ENO1 is a homodimeric soluble protein that encodes a smaller monomeric structural lens protein, tau-crystallin. ENO1 is a glycolytic enzyme expressed in mainly all tissues. ENO1 isoenzyme full length protein is found in the cytoplasm. The shorter protein is formed from another translation start that is restricted to the nucleus, and binds to a component in the c-myc promoter. ENO1 is involved in anaerobic metabolism under hypoxic conditions and plays a role as a cell surface plasminogen receptor during tissue invasion. Irregular expression of Enolase-1 is linked with tumor progression in several cases of breast and lung cancer. Enolase-1 is as an auto antigen associated with Hashimoto's encephalopathy and severe asthma. ENO1 is the target protein of serum anti-endothelial antibody in Behcet's disease.

ENO1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 457 amino acids (1-434 a.a) and having a molecular mass of 24kDa.ENO1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses (). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production ().
UniProt Protein Details:
NCBI Summary:
UniProt Code:P17182
NCBI GenInfo Identifier:13637776
NCBI Gene ID:13806
NCBI Accession:P17182.3
UniProt Secondary Accession:P17182,Q99KT7, Q9DCY7
UniProt Related Accession:P17182
Molecular Weight:47,141 Da
NCBI Full Name:Alpha-enolase
NCBI Synonym Full Names:enolase 1, alpha non-neuron
NCBI Official Symbol:Eno1��
NCBI Official Synonym Symbols:Eno-1; MBP-1; AL022784; 0610008I15��
NCBI Protein Information:alpha-enolase
UniProt Protein Name:Alpha-enolase
UniProt Synonym Protein Names:2-phospho-D-glycerate hydro-lyase; Enolase 1; Non-neural enolase; NNE
Protein Family:Alpha-enolase
UniProt Gene Name:Eno1��
UniProt Entry Name:
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Additional Information

Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P17182
Research Area:
Enzymes
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