Growth Factors & Cytokines Recombinant Proteins

Mouse EGF Recombinant Protein (RPPB0171)

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SKU:
RPPB0171
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P01132
Research Area:
Growth Factors & Cytokines

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Mouse EGF Recombinant Protein
Product Code:RPPB0171
Size:5µg
Species:Mouse
Target:EGF
Synonyms:AI790464, Pro-epidermal growth factor, URG.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:EGF protein solution (0.25mg/ml) contains 10% glycerol, 20mM Tris-HCl (pH 8.0), 0.1M NaCl & 2mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMNSYPGC PSSYDGYCLN GGVCMHIESL DSYTCNCVIG YSGDRCQTRD LRWWELR
Biological Activity:Measured in a cell proliferation assay using mouse Balb/3T3 cell. The ED50 for this effect less or equal to 1ng/ml.

Pro-Epidermal Growth Factor Isoform 1 or EGF, is a globular peptide (77aa residues) which includes three intra molecular disulfide bonds. This protein acts as a growth factor that mediates the growth and proliferation of different epithelial & epidermal cells. Among other processes that EGF is part of are inhibition of gastric secretion and wound healing. EGF is a ligand for class I tyrosine kinase receptor (c-erbB).

EGF Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 77 amino acids (977-1029 a.a) and having a molecular mass of 8.6kDa.EGF is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Hormone; Membrane protein, integral; Ligand, receptor tyrosine kinase

Cellular Component: extracellular space; membrane; lysosomal membrane; plasma membrane; integral to membrane; intracellular

Molecular Function:protein binding; growth factor activity; calcium ion binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; positive regulation of mitosis; positive regulation of transcription, DNA-dependent; regulation of protein transport; positive regulation of MAP kinase activity; regulation of protein secretion; branching morphogenesis of a tube; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; negative regulation of secretion; angiogenesis; regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; positive regulation of granule cell precursor proliferation; positive regulation of DNA binding; STAT protein nuclear translocation

UniProt Code:P01132
NCBI GenInfo Identifier:341940478
NCBI Gene ID:13645
NCBI Accession:P01132.2
UniProt Secondary Accession:P01132,Q569W5, Q6P9J2, E9QNX6,
UniProt Related Accession:P01132
Molecular Weight:133,072 Da
NCBI Full Name:Pro-epidermal growth factor
NCBI Synonym Full Names:epidermal growth factor
NCBI Official Symbol:Egf��
NCBI Official Synonym Symbols:AI790464��
NCBI Protein Information:pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF)
UniProt Protein Name:Pro-epidermal growth factor
Protein Family:EGF-like domain containing protein
UniProt Gene Name:Egf��
UniProt Entry Name:EGF_MOUSE
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system_update_altDatasheetMouse EGF Recombinant Protein EGF is a single-pass type I membrane protein,containing 8 LDL-receptor class B repeats and 9 EGF-like domains. EGF results in cellular prol