Growth Factors & Cytokines Recombinant Proteins

Mouse EGF Protein Recombinant Protein (RPPB0168)

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SKU:
RPPB0168
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P01132
Research Area:
Growth Factors & Cytokines

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Mouse EGF Protein Recombinant Protein
Product Code:RPPB0168
Size:0.5mg
Species:Mouse
Target:EGF Protein
Synonyms:Urogastrone, URG, EGF.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The protein was lyophilized with no additives.
Solubility:It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR
Biological Activity:The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 0.1ng/ml.

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.

Epidermal Growth Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids including 3 intramolecular disulfide-bonds and having a molecular mass of 6 kDa.The EGF is purified by proprietary chromatographic techniques.

UniProt Protein Function:EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Hormone; Membrane protein, integral; Ligand, receptor tyrosine kinase

Cellular Component: extracellular space; membrane; lysosomal membrane; plasma membrane; integral to membrane; intracellular

Molecular Function:protein binding; growth factor activity; calcium ion binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; positive regulation of mitosis; positive regulation of transcription, DNA-dependent; regulation of protein transport; positive regulation of MAP kinase activity; regulation of protein secretion; branching morphogenesis of a tube; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; negative regulation of secretion; angiogenesis; regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; positive regulation of granule cell precursor proliferation; positive regulation of DNA binding; STAT protein nuclear translocation

UniProt Code:P01132
NCBI GenInfo Identifier:341940478
NCBI Gene ID:13645
NCBI Accession:P01132.2
UniProt Secondary Accession:P01132,Q569W5, Q6P9J2, E9QNX6,
UniProt Related Accession:P01132
Molecular Weight:133,072 Da
NCBI Full Name:Pro-epidermal growth factor
NCBI Synonym Full Names:epidermal growth factor
NCBI Official Symbol:Egf��
NCBI Official Synonym Symbols:AI790464��
NCBI Protein Information:pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF)
UniProt Protein Name:Pro-epidermal growth factor
Protein Family:EGF-like domain containing protein
UniProt Gene Name:Egf��
UniProt Entry Name:EGF_MOUSE
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