Cytokines Recombinant Proteins

Mouse DHH (C23II) Recombinant Protein (RPPB0910)

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SKU:
RPPB0910
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
Q61488
Research Area:
Cytokines

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Mouse DHH (C23II) Recombinant Protein
Product Code:RPPB0910
Size:25µg
Species:Mouse
Target:DHH (C23II)
Synonyms:Desert hedgehog protein, DHH, HHG-3, C78960.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a 0.2�m filtered concentrated solution in PBS, pH7.4, 1mM DTT and 0.05% Tween-80.
Solubility:It is recommended to reconstitute the lyophilized DHH (C23II) in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized DHH (C23II) although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DHH (C23II) should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:IIGPGRGPVG RRRYVRKQLV PLLYKQFVPS MPERTLGASG PAEGRVTRGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH IHVSVKADNS LAVRAGG
Biological Activity:The ED50 as determined by its ability to induce alkaline phosphatase production by murine MC3T3-E1 cells is 5-20 �g/ml.

DHH is part of the Hedgehog family which encodes signaling molecules that are involved in regulating morphogenesis. DHH protein is a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. Additionally, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in DHH protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. DHH plays a role both male gonadal differentiation and perineurial development.DHH plays a role in intercellular signaling which is essential for a variety of patterning events during development. DHH functions as a spermatocyte survival factor in the testes & is essential for testes development.

DHH (C23II) Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 177 amino acids and having a molecular mass of 20kDa. The DHH (C23II) is purified by proprietary chromatographic techniques.

UniProt Protein Function:DHH: Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development. Defects in DHH may be the cause of partial gonadal dysgenesis with minifascicular neuropathy 46,XY (PGD). PGD is characterized by the presence of a testis on one side and a streak or an absent gonad at the other, persistence of Muellerian duct structures, and a variable degree of genital ambiguity. Defects in DHH may be the cause of complete pure gonadal dysgenesis 46,XY type (GDXYM); also known as male- limited gonadal dysgenesis 46,XY. GDXYM is a type of hypogonadism in which no functional gonads are present to induce puberty in an externally female person whose karyotype is then found to be XY. The gonads are found to be non-functional streaks. Belongs to the hedgehog family.Protein type: Secreted; Cell development/differentiation; Secreted, signal peptideCellular Component: membrane; plasma membrane; extracellular regionMolecular Function: peptidase activity; protein binding; zinc ion binding; hydrolase activity; metal ion binding; patched binding; calcium ion bindingBiological Process: intein-mediated protein splicing; Leydig cell differentiation; cell-cell signaling; multicellular organismal development; male sex determination; regulation of steroid biosynthetic process; cell communication; proteolysis; spermatid development
UniProt Protein Details:
NCBI Summary:
UniProt Code:Q61488
NCBI GenInfo Identifier:6681181
NCBI Gene ID:13363
NCBI Accession:NP_031883.1
UniProt Secondary Accession:Q61488
UniProt Related Accession:Q61488
Molecular Weight:43,542 Da
NCBI Full Name:desert hedgehog protein
NCBI Synonym Full Names:desert hedgehog
NCBI Official Symbol:Dhh��
NCBI Official Synonym Symbols:C78960��
NCBI Protein Information:desert hedgehog protein; HHG-3
UniProt Protein Name:Desert hedgehog protein
UniProt Synonym Protein Names:HHG-3Cleaved into the following 2 chains:Desert hedgehog protein N-product; Desert hedgehog protein C-product
Protein Family:Desert hedgehog protein
UniProt Gene Name:Dhh��
UniProt Entry Name:DHH_MOUSE
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