Enzymes Recombinant Proteins
Mouse DHFR Recombinant Protein (RPPB1571)
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- SKU:
- RPPB1571
- Product Type:
- Recombinant Protein
- Species:
- Mouse
- Uniprot:
- P00375
- Research Area:
- Enzymes
Description
| Product Name: | Mouse DHFR Recombinant Protein |
| Product Code: | RPPB1571 |
| Size: | 20µg |
| Species: | Mouse |
| Target: | DHFR |
| Synonyms: | Dihydrofolate reductase, DHFR, DHFRP1, AA607882, AI662710, AW555094, 8430436I03Rik. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The DHFR solution (1 mg/ml) contains 20mM Tris-HCl buffer(pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD |
| Biological Activity: | Specific activity is > 0.2 units/mg, in which one unit will convert 1.0 umole of 7,8 �dihydrofloate and beta-NADPH to 5,6,7,8-tetrahydrofloate and beta-NADP per min at pH 6.5 at 25C. |
Dihydrofolate reductase (DHFR) is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, with NADPH as electron donor that can be converted to the kinds of tetrahydrofolate cofactors applied in 1-carbon transfer chemistry. DHFR converts dihydrofolate into tetrahydrofolate, which is a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and specific amino acids. Even though the functional DHFR gene is mapped to chromosome 5, numerous intronless processed pseudogenes or dihydrofolate reductase-like genes are identified on separate chromosomes. DHFR deficiency is associated with megaloblastic anemia. DHFR knockdown plays a role in the anticancer activity of 2-hydroxyoleic acid. DHFR gene insertion/deletion polymorphism is linked to variation in serum and red blood cell folate concentrations in women.
DHFR�Mouse Recombinant fused with a�20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing�207 amino acids (1-187 a.a.) and having a molecular mass of 23.8kDa. The DHFR is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | DHFR: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1. Defects in DHFR are the cause of megaloblastic anemia due to dihydrofolate reductase deficiency (DHFRD). DHFRD is an inborn error of metabolism, characterized by megaloblastic anemia and/or pancytopenia, severe cerebral folate deficiency, and cerebral tetrahydrobiopterin deficiency. Clinical features include variable neurologic symptoms, ranging from severe developmental delay and generalized seizures in infancy, to childhood absence epilepsy with learning difficulties, to lack of symptoms. Belongs to the dihydrofolate reductase family.Protein type: Cofactor and Vitamin Metabolism - folate biosynthesis; EC 1.5.1.3; Oxidoreductase; Cofactor and Vitamin Metabolism - one carbon pool by folateMolecular Function: dihydrofolate reductase activity; dihydrofolic acid binding; drug binding; folic acid binding; methotrexate binding; mRNA binding; NADP bindingBiological Process: axon regeneration; dihydrofolate metabolic process; folic acid metabolic process; positive regulation of nitric-oxide synthase activity; response to methotrexate; tetrahydrobiopterin biosynthetic process; tetrahydrofolate biosynthetic process; tetrahydrofolate metabolic process |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | P00375 |
| NCBI GenInfo Identifier: | 1706542 |
| NCBI Gene ID: | 13361 |
| NCBI Accession: | P00375.3 |
| UniProt Secondary Accession: | P00375,P70693, Q61485, Q61487, Q61579 |
| UniProt Related Accession: | P00375 |
| Molecular Weight: | 21,606 Da |
| NCBI Full Name: | Dihydrofolate reductase |
| NCBI Synonym Full Names: | dihydrofolate reductase |
| NCBI Official Symbol: | Dhfr�� |
| NCBI Official Synonym Symbols: | AA607882; AI662710; AW555094; 8430436I03Rik�� |
| NCBI Protein Information: | dihydrofolate reductase |
| UniProt Protein Name: | Dihydrofolate reductase |
| UniProt Synonym Protein Names: | |
| Protein Family: | Viral dihydrofolate reductase |
| UniProt Gene Name: | Dhfr�� |
| UniProt Entry Name: | DYR_MOUSE |
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