Mouse Cytokine Array (96 targets) (SARB0077)
- SKU:
- SARB0077
- Product Type:
- Protein Array
- Analytes:
- Axl
- Analytes:
- bFGF
- Analytes:
- BLC (CXCL13)
- Analytes:
- CD30 Ligand (TNFSF8)
- Analytes:
- CD30 (TNFRSF8)
- Analytes:
- CD40 (TNFRSF5)
- Analytes:
- CRG-2
- Analytes:
- CTACK (CCL27)
- Analytes:
- CXCL16
- Analytes:
- CD26 (DPPIV)
- Analytes:
- Dtk
- Analytes:
- Eotaxin-1 (CCL11)
- Analytes:
- Eotaxin-2 (MPIF-2/CCL24)
- Analytes:
- E-Selectin
- Analytes:
- Fas Ligand (TNFSF6)
- Analytes:
- Fc gamma RIIB (CD32b)
- Analytes:
- Flt-3 Ligand
- Analytes:
- Fractalkine (CX3CL1)
- Analytes:
- GCSF
- Analytes:
- GITR (TNFRSF18)
- Analytes:
- GM-CSF
- Analytes:
- HGFR
- Analytes:
- ICAM-1 (CD54)
- Analytes:
- IFN-gamma
- Analytes:
- IGFBP-2
- Analytes:
- IGFBP-3
- Analytes:
- IGFBP-5
- Analytes:
- IGFBP-6
- Analytes:
- IGF-1
- Analytes:
- IGF-2
- Analytes:
- IL-1 beta (IL-1 F2)
- Analytes:
- IL-10
- Analytes:
- IL-12 p40/p70
- Analytes:
- IL-12 p70
- Analytes:
- IL-13
- Analytes:
- IL-15
- Analytes:
- IL-17A
- Analytes:
- IL-17 RB
- Analytes:
- IL-1 alpha (IL-1 F1)
- Analytes:
- IL-2
- Analytes:
- IL-3
- Analytes:
- IL-3 R beta
- Analytes:
- IL-4
- Analytes:
- IL-5
- Reactivity:
- Mouse
- Applications:
- Multiplex Array
Description
system_update_altDatasheet
Product Sku: | SARB0077 |
Size: | 2, 4, or 8 |
Species Detected: | Mouse |
Number of Targets Detected: | 96 |
Gene Symbols: | AXL, CCL1, CCL11, CCL12, CCL17, CCL19, CCL2, CCL20, CCL22, CCL24|CCL25, CCL27, CCL3, CCL5, CCL9, CD40, CSF1, CSF2, CSF2RB, CSF3, CX3CL1, CXCL1, CXCL10, CXCL11, CXCL12, CXCL13, CXCL15, CXCL16, CXCL2, CXCL5, CXCL9, DPP4, FASLG, FCGR2B, FGF2, FIGF, FLT1, FLT3LG, FLT4, ICAM1, IFNG, IGF1, IGF2, IGFBP2, IGFBP3, IGFBP5, IGFBP6, IL10, IL12A, IL13, IL15, IL17A, IL17RB, IL1A, IL1B, IL2, IL3, IL4, IL5, IL6, IL7, IL9, KDR, KITLG, LEP, LEPR, MET, MMP2, MMP3, MMP9, PF4, PPBP, RETN, SELE, SELL, SELP, SHH, SPP1, THPO, TIMP1, TIMP2, TNF, TNFRSF11B, TNFRSF18, TNFRSF19, TNFRSF8, TNFSF11, TNFSF8, TPO, TSLP, TYRO3, VCAM1, VEGFA, XCL1 |
Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
Design Principle: | Sandwich-based |
Method of Detection: | Chemiluminescence |
Pathway: | |
Quantitative/Semi-Quantitative: | Semi-Quantitative |
Solid Support: | Membrane |
Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
Hover over target for more information | ||||
---|---|---|---|---|
Axl
| bFGF
| BLC (CXCL13)
| CD30 Ligand (TNFSF8)
| CD30 (TNFRSF8)
|
CD40 (TNFRSF5)
| CRG-2
| CTACK (CCL27)
| CXCL16
| CD26 (DPPIV)
|
Dtk
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| E-Selectin
| Fas Ligand (TNFSF6)
|
Fc gamma RIIB (CD32b)
| Flt-3 Ligand
| Fractalkine (CX3CL1)
| GCSF
| GITR (TNFRSF18)
|
GM-CSF
| HGFR
| ICAM-1 (CD54)
| IFN-gamma
| IGFBP-2
|
IGFBP-3
| IGFBP-5
| IGFBP-6
| IGF-1
| IGF-2
|
IL-1 beta (IL-1 F2)
| IL-10
| IL-12 p40/p70
| IL-12 p70
| IL-13
|
IL-15
| IL-17A
| IL-17 RB
| IL-1 alpha (IL-1 F1)
| IL-2
|
IL-3
| IL-3 R beta
| IL-4
| IL-5
| IL-6
|
IL-7
| IL-9
| I-TAC (CXCL11)
| KC (CXCL1)
| Leptin
|
Leptin R
| LIX
| L-Selectin (CD62L)
| Lungkine (CXCL15)
| Lymphotactin (XCL1)
|
MCP-1 (CCL2)
| MCP-5
| M-CSF
| MDC (CCL22)
| MIG (CXCL9)
|
MIP-1 alpha (CCL3)
| MIP-1 gamma
| MIP-2
| MIP-3 beta (CCL19)
| MIP-3 alpha (CCL20)
|
MMP-2
| MMP-3
| Osteopontin (SPP1)
| Osteoprotegerin (TNFRSF11B)
| Platelet Factor 4 (CXCL4)
|
Pro-MMP-9
| P-Selectin
| RANTES (CCL5)
| Resistin
| SCF
|
SDF-1 alpha (CXCL12 alpha)
| Sonic Hedgehog N-Terminal (Shh-N)
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
| TARC (CCL17)
|
I-309 (TCA-3/CCL1)
| TECK (CCL25)
| TCK-1 (CXCL7)
| TIMP-1
| TIMP-2
|
TNF alpha
| Thrombopoietin (TPO)
| TRANCE (TNFSF11)
| TROY (TNFRSF19)
| TSLP
|
VCAM-1 (CD106)
| VEGF-A
| VEGFR1
| VEGFR2
| VEGFR3
|
VEGF-D
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Mouse Cytokine Antibody Array C1000 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1. | Block membranes |
2. | Incubate with Sample |
3. | Incubate with Biotinylated Detection Antibody Cocktail |
4. | Incubate with HRP-Conjugated Streptavidin |
5. | Incubate with Detection Buffers |
6. | Image with chemiluminescent imaging system |
7. | Perform densitometry and analysis |