Mouse Cytokine Array (62 targets) (SARB0081)

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SKU:
SARB0081
Product Type:
Protein Array
Analytes:
Axl
Analytes:
BLC (CXCL13)
Analytes:
CD30 Ligand (TNFSF8)
Analytes:
CD30 (TNFRSF8)
Analytes:
CD40 (TNFRSF5)
Analytes:
CRG-2
Analytes:
CTACK (CCL27)
Analytes:
CXCL16
Analytes:
Eotaxin-1 (CCL11)
Analytes:
Eotaxin-2 (MPIF-2/CCL24)
Analytes:
Fas Ligand (TNFSF6)
Analytes:
Fractalkine (CX3CL1)
Analytes:
GCSF
Analytes:
GM-CSF
Analytes:
IFN-gamma
Analytes:
IGFBP-3
Analytes:
IGFBP-5
Analytes:
IGFBP-6
Analytes:
IL-1 beta (IL-1 F2)
Analytes:
IL-10
Analytes:
IL-12 p40/p70
Analytes:
IL-12 p70
Analytes:
IL-13
Analytes:
IL-17A
Analytes:
IL-1 alpha (IL-1 F1)
Analytes:
IL-2
Analytes:
IL-3
Analytes:
IL-3 R beta
Analytes:
IL-4
Analytes:
IL-5
Analytes:
IL-6
Analytes:
IL-9
Analytes:
KC (CXCL1)
Analytes:
Leptin
Analytes:
Leptin R
Analytes:
LIX
Analytes:
L-Selectin (CD62L)
Analytes:
Lymphotactin (XCL1)
Analytes:
MCP-1 (CCL2)
Analytes:
MCP-5
Analytes:
M-CSF
Analytes:
MIG (CXCL9)
Analytes:
MIP-1 alpha (CCL3)
Analytes:
MIP-1 gamma
Reactivity:
Mouse
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0081
Size:2, 4, or 8
Species Detected:Mouse
Number of Targets Detected:62
Gene Symbols:AXL, CCL1, CCL11, CCL12, CCL17, CCL19, CCL2, CCL20, CCL24, CCL25|CCL27, CCL3, CCL5, CCL9, CD40, CSF1, CSF2, CSF2RB, CSF3, CX3CL1, CXCL1, CXCL10, CXCL12, CXCL13, CXCL16, CXCL2, CXCL5, CXCL9, FASLG, IFNG, IGFBP3, IGFBP5, IGFBP6, IL10, IL12A, IL13, IL17A, IL1A, IL1B, IL2, IL3, IL4, IL5, IL6, IL9, KITLG, LEP, LEPR, PF4, SELL, SELP, THPO, TIMP1, TNF, TNFRSF8, TNFSF8, TPO, VCAM1, VEGFA, XCL1
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Axl
  • Gene Symbol: Axl
  • Gene ID: N/A
  • Accession #: Q00993
BLC (CXCL13)
  • Gene Symbol: CXCL13
  • Gene ID: N/A
  • Accession #: O55038
CD30 Ligand (TNFSF8)
  • Gene Symbol: CD30LG
  • Gene ID: N/A
  • Accession #: P32972
CD30 (TNFRSF8)
  • Gene Symbol: CD30
  • Gene ID: N/A
  • Accession #: Q60846
CD40 (TNFRSF5)
  • Gene Symbol: CD40
  • Gene ID: N/A
  • Accession #: Q99NE2
CRG-2
  • Gene Symbol: Cxcl10
  • Gene ID: N/A
  • Accession #: P17515
CTACK (CCL27)
  • Gene Symbol: CCL27
  • Gene ID: N/A
  • Accession #: Q9Z1X0
CXCL16
  • Gene Symbol: CXCL16
  • Gene ID: N/A
  • Accession #: Q5F2D5
Eotaxin-1 (CCL11)
  • Gene Symbol: CCL11
  • Gene ID: N/A
  • Accession #: P48298
Eotaxin-2 (MPIF-2/CCL24)
  • Gene Symbol: Ccl24
  • Gene ID: N/A
  • Accession #: Q9JKC0
Fas Ligand (TNFSF6)
  • Gene Symbol: FASLG
  • Gene ID: N/A
  • Accession #: P41047
Fractalkine (CX3CL1)
  • Gene Symbol: CX3CL1
  • Gene ID: N/A
  • Accession #: O35188
GCSF
  • Gene Symbol: CSF3
  • Gene ID: N/A
  • Accession #: P09920
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: N/A
  • Accession #: P26955
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: N/A
  • Accession #: P01580
IGFBP-3
  • Gene Symbol: IGFBP3
  • Gene ID: N/A
  • Accession #: P47878
IGFBP-5
  • Gene Symbol: IGFBP5
  • Gene ID: N/A
  • Accession #: Q07079
IGFBP-6
  • Gene Symbol: IGFBP6
  • Gene ID: N/A
  • Accession #: Q91X24
IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: N/A
  • Accession #: P10749
IL-10
  • Gene Symbol: IL10
  • Gene ID: N/A
  • Accession #: Q0VBJ1
IL-12 p40/p70
  • Gene Symbol: IL12A; IL12B
  • Gene ID: N/A
  • Accession #: P43432
IL-12 p70
  • Gene Symbol: IL12A
  • Gene ID: N/A
  • Accession #: P43431
IL-13
  • Gene Symbol: IL13
  • Gene ID: N/A
  • Accession #: P20109
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: N/A
  • Accession #: Q60971
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: N/A
  • Accession #: P01582
IL-2
  • Gene Symbol: IL2
  • Gene ID: N/A
  • Accession #: Q791T3
IL-3
  • Gene Symbol: IL3
  • Gene ID: N/A
  • Accession #: P01586
IL-3 R beta
  • Gene Symbol: Csf2rb
  • Gene ID: N/A
  • Accession #: P26955
IL-4
  • Gene Symbol: IL4
  • Gene ID: N/A
  • Accession #: P07750
IL-5
  • Gene Symbol: IL5
  • Gene ID: N/A
  • Accession #: P04401
IL-6
  • Gene Symbol: IL6
  • Gene ID: N/A
  • Accession #: P08505
IL-9
  • Gene Symbol: IL9
  • Gene ID: N/A
  • Accession #: P15247
KC (CXCL1)
  • Gene Symbol: CXCL1
  • Gene ID: N/A
  • Accession #: P12850
Leptin
  • Gene Symbol: LEP
  • Gene ID: N/A
  • Accession #: P41160
Leptin R
  • Gene Symbol: LEPR
  • Gene ID: N/A
  • Accession #: Q61215
LIX
  • Gene Symbol: Cxcl5
  • Gene ID: N/A
  • Accession #: P50228
L-Selectin (CD62L)
  • Gene Symbol: SELL
  • Gene ID: N/A
  • Accession #: Q3TCF3
Lymphotactin (XCL1)
  • Gene Symbol: XCL1
  • Gene ID: N/A
  • Accession #: P47993
MCP-1 (CCL2)
  • Gene Symbol: CCL2
  • Gene ID: N/A
  • Accession #: Q9QYD7
MCP-5
  • Gene Symbol: Ccl12
  • Gene ID: N/A
  • Accession #: Q9QYD6
M-CSF
  • Gene Symbol: CSF1
  • Gene ID: N/A
  • Accession #: P07141
MIG (CXCL9)
  • Gene Symbol: CXCL9
  • Gene ID: N/A
  • Accession #: P18340
MIP-1 alpha (CCL3)
  • Gene Symbol: CCL3
  • Gene ID: N/A
  • Accession #: P14096
MIP-1 gamma
  • Gene Symbol: Ccl9
  • Gene ID: N/A
  • Accession #: Q5QNW2
MIP-2
  • Gene Symbol: Cxcl2
  • Gene ID: N/A
  • Accession #: P10889
MIP-3 beta (CCL19)
  • Gene Symbol: CCL19
  • Gene ID: N/A
  • Accession #: O70460
MIP-3 alpha (CCL20)
  • Gene Symbol: CCL20
  • Gene ID: N/A
  • Accession #: O89093
Platelet Factor 4 (CXCL4)
  • Gene Symbol: PF4, CXCL4, SCYB4
  • Gene ID: N/A
  • Accession #: Q9Z126
P-Selectin
  • Gene Symbol: SELP
  • Gene ID: N/A
  • Accession #: Q01102
RANTES (CCL5)
  • Gene Symbol: CCL5
  • Gene ID: N/A
  • Accession #: P30882
SCF
  • Gene Symbol: KITLG
  • Gene ID: N/A
  • Accession #: P20826
SDF-1 alpha (CXCL12 alpha)
  • Gene Symbol: CXCL12
  • Gene ID: N/A
  • Accession #: P40224
TNF RI (TNFRSF1A)
  • Gene Symbol: TNFRSF1A
  • Gene ID: N/A
  • Accession #: P25118
TNF RII (TNFRSF1B)
  • Gene Symbol: TNFRSF1B
  • Gene ID: N/A
  • Accession #: O88734
TARC (CCL17)
  • Gene Symbol: CCL17
  • Gene ID: N/A
  • Accession #: Q9WUZ6
I-309 (TCA-3/CCL1)
  • Gene Symbol: CCL1
  • Gene ID: N/A
  • Accession #: P14098
TECK (CCL25)
  • Gene Symbol: CCL25
  • Gene ID: N/A
  • Accession #: O35903
TIMP-1
  • Gene Symbol: TIMP1
  • Gene ID: N/A
  • Accession #: P12032
TNF alpha
  • Gene Symbol: TNF
  • Gene ID: N/A
  • Accession #: Q62326
Thrombopoietin (TPO)
  • Gene Symbol: THPO
  • Gene ID: N/A
  • Accession #: P40226
VCAM-1 (CD106)
  • Gene Symbol: VCAM1
  • Gene ID: N/A
  • Accession #: P29533
VEGF-A
  • Gene Symbol: VEGFA
  • Gene ID: N/A
  • Accession #: Q00731
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Mouse Cytokine Antibody Array C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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