Mouse Cytokine Array (48 targets) (SARB0083)

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SKU:
SARB0083

Description

system_update_altDatasheet
Product Sku:SARB0083
Size:2, 4, or 8
Species Detected:Mouse
Number of Targets Detected:48
Gene Symbols:ACE, AGER, AREG, CCL21, CD27, CD36, CD40LG, CD70, CDH1, CHRD|CTF1, CTLA4, DCN, DKK1, EGF, ENG, EPGN, EREG, F11R, GAS1, GAS6, GZMB, HGF, IFNL2, IL11, IL17B, IL17F, IL1RL1, IL1RN, IL20, IL21, IL25, IL2RA, IL6R, LGALS1, MADCAM1, MFGE8, MME, PRL, PTX3, SLPI, SPINT1, TNFRSF13B, TNFRSF9, TNFSF12, TNFSF18, TNFSF9, TREM1
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
4-1BB (TNFRSF9/CD137)
  • Gene Symbol: TNFRSF9
  • Gene ID: N/A
  • Accession #: P20334
6Ckine (CCL21)
  • Gene Symbol: CCL21
  • Gene ID: N/A
  • Accession #: P86792
ACE
  • Gene Symbol: ACE
  • Gene ID: N/A
  • Accession #: P22967
ALK-1
  • Gene Symbol: SLPI
  • Gene ID: N/A
  • Accession #: O09081
Amphiregulin
  • Gene Symbol: AREG
  • Gene ID: N/A
  • Accession #: P31955
Cardiotrophin-1 (CT-1)
  • Gene Symbol: CTF1
  • Gene ID: N/A
  • Accession #: Q60753
CD27 (TNFRSF7)
  • Gene Symbol: CD27
  • Gene ID: N/A
  • Accession #: P41272
CD27 Ligand (TNFSF7)
  • Gene Symbol: CD70
  • Gene ID: N/A
  • Accession #: P32970
CD36 (SR-B3)
  • Gene Symbol: CD36
  • Gene ID: N/A
  • Accession #: Q08857
CD40 Ligand (TNFSF5)
  • Gene Symbol: CD40LG
  • Gene ID: N/A
  • Accession #: P27548
Chordin
  • Gene Symbol: CHRD
  • Gene ID: N/A
  • Accession #: Q9Z0E2
CTLA-4 (CD152)
  • Gene Symbol: Ctla4
  • Gene ID: N/A
  • Accession #: Q9QZZ7
Decorin
  • Gene Symbol: DCN
  • Gene ID: N/A
  • Accession #: P28654
DKK-1
  • Gene Symbol: DKK1
  • Gene ID: N/A
  • Accession #: Q80UL5
E-Cadherin
  • Gene Symbol: CDH1
  • Gene ID: N/A
  • Accession #: P09803
EGF
  • Gene Symbol: EGF
  • Gene ID: N/A
  • Accession #: E9QNX6
Endoglin (CD105)
  • Gene Symbol: ENG
  • Gene ID: N/A
  • Accession #: Q63961
Epigen
  • Gene Symbol: EPGN
  • Gene ID: N/A
  • Accession #: Q8CEX5
Epiregulin
  • Gene Symbol: EREG
  • Gene ID: N/A
  • Accession #: Q61521
Galectin-1
  • Gene Symbol: LGALS1
  • Gene ID: N/A
  • Accession #: P17601
Gas 1
  • Gene Symbol: GAS1
  • Gene ID: N/A
  • Accession #: Q01721
Gas 6
  • Gene Symbol: Gas6
  • Gene ID: N/A
  • Accession #: Q99K57
GITR Ligand (TNFSF18)
  • Gene Symbol: TNFSF18
  • Gene ID: N/A
  • Accession #: Q7TS55
Granzyme B
  • Gene Symbol: GZMB
  • Gene ID: N/A
  • Accession #: Q3V2P1
HAI-1
  • Gene Symbol: SPINT1
  • Gene ID: N/A
  • Accession #: Q9R097
HGF
  • Gene Symbol: HGF
  • Gene ID: N/A
  • Accession #: Q53WS5
IL-1 R4 (ST2)
  • Gene Symbol: IL1RL1
  • Gene ID: N/A
  • Accession #: Q05208
IL-11
  • Gene Symbol: IL11
  • Gene ID: N/A
  • Accession #: P47873
IL-17B
  • Gene Symbol: IL17B
  • Gene ID: N/A
  • Accession #: Q99MY3
IL-17E (IL-25)
  • Gene Symbol: IL25
  • Gene ID: N/A
  • Accession #: Q8VHH8
IL-17F
  • Gene Symbol: IL17F
  • Gene ID: N/A
  • Accession #: Q7TNI7
IL-1 RA (IL-1 F3)
  • Gene Symbol: IL1RN
  • Gene ID: N/A
  • Accession #: P25085
IL-2 R alpha
  • Gene Symbol: IL2RA
  • Gene ID: N/A
  • Accession #: Q61731
IL-20
  • Gene Symbol: IL20
  • Gene ID: N/A
  • Accession #: Q9JKV9
IL-21
  • Gene Symbol: IL21
  • Gene ID: N/A
  • Accession #: E9PX58
IL-28A (IFN-lambda 2)
  • Gene Symbol: IFNL2
  • Gene ID: N/A
  • Accession #: Q4VK74
IL-6 R
  • Gene Symbol: IL6R
  • Gene ID: N/A
  • Accession #: P22272
JAM-A (CD321/F11R)
  • Gene Symbol: F11R
  • Gene ID: N/A
  • Accession #: Q8VC39
MAdCAM-1
  • Gene Symbol: MADCAM1
  • Gene ID: N/A
  • Accession #: Q61278
MFG-E8
  • Gene Symbol: Mfge8
  • Gene ID: N/A
  • Accession #: Q9R1X9
Neprilysin
  • Gene Symbol: MME
  • Gene ID: N/A
  • Accession #: Q6NXX5
Pentraxin-3 (TSG-14)
  • Gene Symbol: PTX3
  • Gene ID: N/A
  • Accession #: P48759
Prolactin
  • Gene Symbol: PRL
  • Gene ID: N/A
  • Accession #: P06879
RAGE
  • Gene Symbol: MOK
  • Gene ID: N/A
  • Accession #: Q9WVS4
TACI (TNFRSF13B)
  • Gene Symbol: TNFRSF13B
  • Gene ID: N/A
  • Accession #: Q9DBZ3
TREM-1
  • Gene Symbol: TREM1
  • Gene ID: N/A
  • Accession #: Q3TAL7
TWEAK (TNFSF12)
  • Gene Symbol: TNFSF12
  • Gene ID: N/A
  • Accession #: O54907
TWEAK R (TNFRSF12)
  • Gene Symbol: TNFRSF12A
  • Gene ID: N/A
  • Accession #: Q9CR75
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Mouse Cytokine Antibody Array C5 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product Type:
Protein Array
Analytes:
4-1BB (TNFRSF9/CD137)
Analytes:
6Ckine (CCL21)
Analytes:
ACE
Analytes:
ALK-1
Analytes:
Amphiregulin
Analytes:
Cardiotrophin-1 (CT-1)
Analytes:
CD27 (TNFRSF7)
Analytes:
CD27 Ligand (TNFSF7)
Analytes:
CD36 (SR-B3)
Analytes:
CD40 Ligand (TNFSF5)
Analytes:
Chordin
Analytes:
CTLA-4 (CD152)
Analytes:
Decorin
Analytes:
DKK-1
Analytes:
E-Cadherin
Analytes:
EGF
Analytes:
Endoglin (CD105)
Analytes:
Epigen
Analytes:
Epiregulin
Analytes:
Galectin-1
Analytes:
Gas 1
Analytes:
Gas 6
Analytes:
GITR Ligand (TNFSF18)
Analytes:
Granzyme B
Analytes:
HAI-1
Analytes:
HGF
Analytes:
IL-1 R4 (ST2)
Analytes:
IL-11
Analytes:
IL-17B
Analytes:
IL-17E (IL-25)
Analytes:
IL-17F
Analytes:
IL-1 RA (IL-1 F3)
Analytes:
IL-2 R alpha
Analytes:
IL-20
Analytes:
IL-21
Analytes:
IL-28A (IFN-lambda 2)
Analytes:
IL-6 R
Analytes:
JAM-A (CD321/F11R)
Analytes:
MAdCAM-1
Analytes:
MFG-E8
Analytes:
Neprilysin
Analytes:
Pentraxin-3 (TSG-14)
Analytes:
Prolactin
Analytes:
RAGE
Reactivity:
Mouse
Applications:
Multiplex Array
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