Mouse Cytokine Array (40 targets) (SARB0085)
- SKU:
- SARB0085
- Product Type:
- Protein Array
- Analytes:
- CD80 (B7-1)
- Analytes:
- BAFF R
- Analytes:
- Betacellulin (BTC)
- Analytes:
- C5a
- Analytes:
- CCL6
- Analytes:
- CD48 (SLAMF2)
- Analytes:
- CD6
- Analytes:
- Chemerin
- Analytes:
- Clusterin
- Analytes:
- Lungkine (CXCL15)
- Analytes:
- Cystatin C
- Analytes:
- DAN
- Analytes:
- DLL4
- Analytes:
- EDAR
- Analytes:
- Endocan
- Analytes:
- Fetuin A
- Analytes:
- H60
- Analytes:
- IL-33 (IL-1 F11)
- Analytes:
- IL-7 R alpha
- Analytes:
- Kremen-1
- Analytes:
- Limitin
- Analytes:
- Lipocalin-2 (NGAL)
- Analytes:
- LOX-1
- Analytes:
- Marapsin
- Analytes:
- MBL-2
- Analytes:
- Meteorin
- Analytes:
- Nope
- Analytes:
- NOV (CCN3)
- Analytes:
- Osteoactivin (GPNMB)
- Analytes:
- OX40 Ligand
- Analytes:
- P-Cadherin
- Analytes:
- Periostin
- Analytes:
- PLGF-2
- Analytes:
- Progranulin
- Analytes:
- Prostasin
- Analytes:
- Renin 1
- Analytes:
- Testican 3 (SPOCK3)
- Analytes:
- TIM-1 (KIM-1)
- Analytes:
- TRAIL (TNFSF10)
- Analytes:
- Tryptase epsilon
- Reactivity:
- Mouse
- Applications:
- Multiplex Array
Description
system_update_altDatasheet
| Product Sku: | SARB0085 |
| Size: | 2, 4, or 8 |
| Species Detected: | Mouse |
| Number of Targets Detected: | 40 |
| Gene Symbols: | AHSG, BTC, C5, CCL6, CD48, CD6, CD80, CDH3, CLU, CST3|CXCL15, DLL4, EDAR, ESM1, GPNMB, GRN, H60A, HAVCR1, IFNA11, IGDCC4, IL33, IL7R, KREMEN1, LCN2, MBL2, METRN, NOV, OLR1, PARN, PGF, POSTN, PRSS22, PRSS27, PRSS8, RARRES2, REN1, SPOCK3, TNFRSF13C, TNFSF10, TNFSF4 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
CD80 (B7-1)
| BAFF R
| Betacellulin (BTC)
| C5a
| CCL6
|
CD48 (SLAMF2)
| CD6
| Chemerin
| Clusterin
| Lungkine (CXCL15)
|
Cystatin C
| DAN
| DLL4
| EDAR
| Endocan
|
Fetuin A
| H60
| IL-33 (IL-1 F11)
| IL-7 R alpha
| Kremen-1
|
Limitin
| Lipocalin-2 (NGAL)
| LOX-1
| Marapsin
| MBL-2
|
Meteorin
| Nope
| NOV (CCN3)
| Osteoactivin (GPNMB)
| OX40 Ligand
|
P-Cadherin
| Periostin
| PLGF-2
| Progranulin
| Prostasin
|
Renin 1
| Testican 3 (SPOCK3)
| TIM-1 (KIM-1)
| TRAIL (TNFSF10)
| Tryptase epsilon
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Mouse Cytokine Array C7 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 4-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |