Mouse Cytokine Array (40 targets) (SARB0085)

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SKU:
SARB0085

Description

system_update_altDatasheet
Product Sku:SARB0085
Size:2, 4, or 8
Species Detected:Mouse
Number of Targets Detected:40
Gene Symbols:AHSG, BTC, C5, CCL6, CD48, CD6, CD80, CDH3, CLU, CST3|CXCL15, DLL4, EDAR, ESM1, GPNMB, GRN, H60A, HAVCR1, IFNA11, IGDCC4, IL33, IL7R, KREMEN1, LCN2, MBL2, METRN, NOV, OLR1, PARN, PGF, POSTN, PRSS22, PRSS27, PRSS8, RARRES2, REN1, SPOCK3, TNFRSF13C, TNFSF10, TNFSF4
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
CD80 (B7-1)
  • Gene Symbol: CD80
  • Gene ID: N/A
  • Accession #: Q00609
BAFF R
  • Gene Symbol: TNFRSF13C
  • Gene ID: N/A
  • Accession #: Q9D8D0
Betacellulin (BTC)
  • Gene Symbol: BTC
  • Gene ID: N/A
  • Accession #: Q05928
C5a
  • Gene Symbol: C5
  • Gene ID: N/A
  • Accession #: P06684
CCL6
  • Gene Symbol: CCL6
  • Gene ID: N/A
  • Accession #: P27784
CD48 (SLAMF2)
  • Gene Symbol: CD48
  • Gene ID: N/A
  • Accession #: Q545K2
CD6
  • Gene Symbol: CD6
  • Gene ID: N/A
  • Accession #: Q61003
Chemerin
  • Gene Symbol: RARRES2
  • Gene ID: N/A
  • Accession #: Q8CHU8
Clusterin
  • Gene Symbol: CLU
  • Gene ID: N/A
  • Accession #: Q06890
Lungkine (CXCL15)
  • Gene Symbol: CXCL15
  • Gene ID: N/A
  • Accession #: Q3UQ15
Cystatin C
  • Gene Symbol: CST3
  • Gene ID: N/A
  • Accession #: Q544Y0
DAN
  • Gene Symbol: PARN
  • Gene ID: N/A
  • Accession #: Q8C7N6
DLL4
  • Gene Symbol: DLL4
  • Gene ID: N/A
  • Accession #: Q9JI71
EDAR
  • Gene Symbol: EDAR
  • Gene ID: N/A
  • Accession #: Q9DC43
Endocan
  • Gene Symbol: ESM1
  • Gene ID: N/A
  • Accession #: Q9QYY7
Fetuin A
  • Gene Symbol: AHSG
  • Gene ID: N/A
  • Accession #: P29699
H60
  • Gene Symbol: H60a
  • Gene ID: N/A
  • Accession #: Q3TDZ7
IL-33 (IL-1 F11)
  • Gene Symbol: IL33
  • Gene ID: N/A
  • Accession #: Q8BVZ5
IL-7 R alpha
  • Gene Symbol: IL7R
  • Gene ID: N/A
  • Accession #: Q9R0C1
Kremen-1
  • Gene Symbol: KREMEN1
  • Gene ID: N/A
  • Accession #: Q99N43
Limitin
  • Gene Symbol: IFNZ
  • Gene ID: N/A
  • Accession #: N/A
Lipocalin-2 (NGAL)
  • Gene Symbol: LCN2
  • Gene ID: N/A
  • Accession #: Q3UE34
LOX-1
  • Gene Symbol: OLR1
  • Gene ID: N/A
  • Accession #: Q9EQ09
Marapsin
  • Gene Symbol: PRSS27
  • Gene ID: N/A
  • Accession #: Q14A25
MBL-2
  • Gene Symbol: MBL2
  • Gene ID: N/A
  • Accession #: P41317
Meteorin
  • Gene Symbol: METRN
  • Gene ID: N/A
  • Accession #: Q8CI64
Nope
  • Gene Symbol: IGDCC4
  • Gene ID: N/A
  • Accession #: Q9EQS9
NOV (CCN3)
  • Gene Symbol: NOV
  • Gene ID: N/A
  • Accession #: Q64299
Osteoactivin (GPNMB)
  • Gene Symbol: GPNMB
  • Gene ID: N/A
  • Accession #: Q99P91
OX40 Ligand
  • Gene Symbol: TNFSF4
  • Gene ID: 7292
  • Accession #: N/A
P-Cadherin
  • Gene Symbol: CDH3
  • Gene ID: N/A
  • Accession #: Q8BSL6
Periostin
  • Gene Symbol: POSTN
  • Gene ID: N/A
  • Accession #: Q62009
PLGF-2
  • Gene Symbol: Pgf
  • Gene ID: N/A
  • Accession #: P49764
Progranulin
  • Gene Symbol: GRN
  • Gene ID: N/A
  • Accession #: P28798
Prostasin
  • Gene Symbol: PRSS8
  • Gene ID: N/A
  • Accession #: Q99L44
Renin 1
  • Gene Symbol: REN
  • Gene ID: N/A
  • Accession #: P06281
Testican 3 (SPOCK3)
  • Gene Symbol: SPOCK3
  • Gene ID: 72902
  • Accession #: Q8BKV0
TIM-1 (KIM-1)
  • Gene Symbol: HAVCR1
  • Gene ID: N/A
  • Accession #: Q8VIM1
TRAIL (TNFSF10)
  • Gene Symbol: TNFSF10
  • Gene ID: N/A
  • Accession #: P50592
Tryptase epsilon
  • Gene Symbol: PRSS22
  • Gene ID: N/A
  • Accession #: Q9ER10
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Mouse Cytokine Array C7 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 4-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product Type:
Protein Array
Analytes:
CD80 (B7-1)
Analytes:
BAFF R
Analytes:
Betacellulin (BTC)
Analytes:
C5a
Analytes:
CCL6
Analytes:
CD48 (SLAMF2)
Analytes:
CD6
Analytes:
Chemerin
Analytes:
Clusterin
Analytes:
Lungkine (CXCL15)
Analytes:
Cystatin C
Analytes:
DAN
Analytes:
DLL4
Analytes:
EDAR
Analytes:
Endocan
Analytes:
Fetuin A
Analytes:
H60
Analytes:
IL-33 (IL-1 F11)
Analytes:
IL-7 R alpha
Analytes:
Kremen-1
Analytes:
Limitin
Analytes:
Lipocalin-2 (NGAL)
Analytes:
LOX-1
Analytes:
Marapsin
Analytes:
MBL-2
Analytes:
Meteorin
Analytes:
Nope
Analytes:
NOV (CCN3)
Analytes:
Osteoactivin (GPNMB)
Analytes:
OX40 Ligand
Analytes:
P-Cadherin
Analytes:
Periostin
Analytes:
PLGF-2
Analytes:
Progranulin
Analytes:
Prostasin
Analytes:
Renin 1
Analytes:
Testican 3 (SPOCK3)
Analytes:
TIM-1 (KIM-1)
Analytes:
TRAIL (TNFSF10)
Analytes:
Tryptase epsilon
Reactivity:
Mouse
Applications:
Multiplex Array
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