Mouse Cytokine Array (34 targets) (SARB0082)

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SKU:
SARB0082
Product Type:
Protein Array
Analytes:
bFGF
Analytes:
CD26 (DPPIV)
Analytes:
Dtk
Analytes:
E-Selectin
Analytes:
Fc gamma RIIB (CD32b)
Analytes:
Flt-3 Ligand
Analytes:
GITR (TNFRSF18)
Analytes:
HGFR
Analytes:
ICAM-1 (CD54)
Analytes:
IGFBP-2
Analytes:
IGF-1
Analytes:
IGF-2
Analytes:
IL-15
Analytes:
IL-17 RB
Analytes:
IL-7
Analytes:
I-TAC (CXCL11)
Analytes:
Lungkine (CXCL15)
Analytes:
MDC (CCL22)
Analytes:
MMP-2
Analytes:
MMP-3
Analytes:
Osteopontin (SPP1)
Analytes:
Osteoprotegerin (TNFRSF11B)
Analytes:
Pro-MMP-9
Analytes:
Resistin
Analytes:
Sonic Hedgehog N-Terminal (Shh-N)
Analytes:
TCK-1 (CXCL7)
Analytes:
TIMP-2
Analytes:
TRANCE (TNFSF11)
Analytes:
TROY (TNFRSF19)
Analytes:
TSLP
Analytes:
VEGFR1
Analytes:
VEGFR2
Analytes:
VEGFR3
Analytes:
VEGF-D
Reactivity:
Mouse
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0082
Size:2, 4, or 8
Species Detected:Mouse
Number of Targets Detected:34
Gene Symbols:CCL22, CXCL11, CXCL15, DPP4, FCGR2B, FGF2, FIGF, FLT1, FLT3LG, FLT4|ICAM1, IGF1, IGF2, IGFBP2, IL15, IL17RB, IL7, KDR, MET, MMP2, MMP3, MMP9, PPBP, RETN, SELE, SHH, SPP1, TIMP2, TNFRSF11B, TNFRSF18, TNFRSF19, TNFSF11, TSLP, TYRO3
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
bFGF
  • Gene Symbol: FGF2
  • Gene ID: N/A
  • Accession #: P15655
CD26 (DPPIV)
  • Gene Symbol: DPP4
  • Gene ID: N/A
  • Accession #: P28843
Dtk
  • Gene Symbol: TYRO3
  • Gene ID: N/A
  • Accession #: O09080
E-Selectin
  • Gene Symbol: SELE
  • Gene ID: N/A
  • Accession #: Q00690
Fc gamma RIIB (CD32b)
  • Gene Symbol: FCGR2B
  • Gene ID: N/A
  • Accession #: P08101
Flt-3 Ligand
  • Gene Symbol: FLT3LG
  • Gene ID: N/A
  • Accession #: P49772
GITR (TNFRSF18)
  • Gene Symbol: TNFRSF18
  • Gene ID: N/A
  • Accession #: Q9JKR2
HGFR
  • Gene Symbol: MET
  • Gene ID: N/A
  • Accession #: D3YVY2
ICAM-1 (CD54)
  • Gene Symbol: ICAM1
  • Gene ID: N/A
  • Accession #: P13597
IGFBP-2
  • Gene Symbol: IGFBP2
  • Gene ID: N/A
  • Accession #: P47877
IGF-1
  • Gene Symbol: IGF1
  • Gene ID: N/A
  • Accession #: P05018
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: N/A
  • Accession #: P09535
IL-15
  • Gene Symbol: IL15
  • Gene ID: N/A
  • Accession #: Q1AHQ7
IL-17 RB
  • Gene Symbol: IL17RB
  • Gene ID: N/A
  • Accession #: Q9JIP3
IL-7
  • Gene Symbol: IL7
  • Gene ID: N/A
  • Accession #: P10168
I-TAC (CXCL11)
  • Gene Symbol: CXCL11
  • Gene ID: N/A
  • Accession #: Q9JHH5
Lungkine (CXCL15)
  • Gene Symbol: CXCL15
  • Gene ID: N/A
  • Accession #: Q3UQ15
MDC (CCL22)
  • Gene Symbol: CCL22
  • Gene ID: N/A
  • Accession #: O88430
MMP-2
  • Gene Symbol: MMP2
  • Gene ID: N/A
  • Accession #: P33434
MMP-3
  • Gene Symbol: MMP3
  • Gene ID: N/A
  • Accession #: P28862
Osteopontin (SPP1)
  • Gene Symbol: SPP1
  • Gene ID: N/A
  • Accession #: P19008
Osteoprotegerin (TNFRSF11B)
  • Gene Symbol: TNFRSF11B
  • Gene ID: N/A
  • Accession #: O70202
Pro-MMP-9
  • Gene Symbol: Mmp9
  • Gene ID: N/A
  • Accession #: Q80XI8
Resistin
  • Gene Symbol: RETN
  • Gene ID: N/A
  • Accession #: Q99P87
Sonic Hedgehog N-Terminal (Shh-N)
  • Gene Symbol: SHH
  • Gene ID: N/A
  • Accession #: N/A
TCK-1 (CXCL7)
  • Gene Symbol: Ppbp
  • Gene ID: N/A
  • Accession #: Q9EQI5
TIMP-2
  • Gene Symbol: TIMP2
  • Gene ID: N/A
  • Accession #: Q6GXA8
TRANCE (TNFSF11)
  • Gene Symbol: TNFSF11
  • Gene ID: N/A
  • Accession #: Q9R1Y0
TROY (TNFRSF19)
  • Gene Symbol: TNFRSF19
  • Gene ID: N/A
  • Accession #: Q80T13
TSLP
  • Gene Symbol: TSLP
  • Gene ID: N/A
  • Accession #: Q9JIE6
VEGFR1
  • Gene Symbol: Flt1
  • Gene ID: N/A
  • Accession #: Q61517
VEGFR2
  • Gene Symbol: KDR
  • Gene ID: N/A
  • Accession #: Q3UQZ6
VEGFR3
  • Gene Symbol: FLT4
  • Gene ID: N/A
  • Accession #: P35917
VEGF-D
  • Gene Symbol: FIGF
  • Gene ID: N/A
  • Accession #: A2AIH3
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Mouse Cytokine Antibody Array C4 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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