Mouse Cell Signalling ELISA Kits 2
Mouse COR(Cortisol) ELISA Kit
- SKU:
- MOFI01342
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Sensitivity:
- 0.234ng/ml
- Range:
- 0.391-25ng/ml
- ELISA Type:
- Competitive
- Synonyms:
- Cortisol
- Reactivity:
- Mouse
Description
Kit Name
Cortisol, a vital hormone belonging to the glucocorticoid class, plays a crucial role in regulating various physiological processes in mammals. The Mouse Cortisol ELISA kits provide researchers and clinicians with a reliable tool to quantitatively assess cortisol concentrations in a wide range of applications.
Key Features
| Save Time | Pre-coated 96 well plate | |
| Quick Start | Kit includes all necessary reagents | |
| Publication Ready | Reproducible and reliable results |
Overview
| Product Name: | Mouse COR(Cortisol) ELISA Kit |
| Product Code: | MOFI01342 |
| Size: | 96 Assays |
| Alias: | Cortisol |
| Detection Method: | Competitive ELISA |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Mouse COR concentrations in serum plasma and other biological fluids. |
| Sensitivity: | 0.234ng/ml |
| Range: | 0.391-25ng/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
Additional Information
| Recovery | Matrices listed below were spiked with certain level of Mouse COR and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse COR in samples. Not Available |
| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse COR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Not Available |
| Intra Assay | CV < 8% |
| Inter Assay | CV < 10% |
Kit Components
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8x12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/ -20°C |
| Sample/Standard Dlution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody (Concentrated) | 60ul | 4°C (Protection from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate (SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protection from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer (25X) | 30ml | 4°C |
| Plate Sealer | 5 | - |
Other materials required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
Protocol
*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Equilibrate the TMB substrate for at least 30 min at 37°C beforeuse. When diluting samples and reagents, they must be mixed completely andevenly. It is recommended to plot a standard curve for each test.
| Step | Procedure |
| 1. | Set standard, test sample and control (zero) wells on the pre-coatedplate respectively, and then, record their positions. It isrecommended to measure each standard and sample in duplicate. Washplate 2 times before adding standard, sample and control (zero) wells! |
| 2. | Add Sample and Biotin-detection antibody: Add 50µL of Standard, Blank or Sample per well. The blankwell is added with Sample Dilution Buffer. Immediately add 50 µL of biotin-labelled antibody workingsolution to each well. Cover with the plate sealer provided. Gently tap the plate to ensure thoroughmixing. Incubate for 45 minutes at 37°C. (Solutions are added to the bottom of micro-ELISA platewell, avoid touching plate walls and foaming). |
| 3. | Wash: Aspirate each well and wash, repeating the process three timesWash by filling each well with Wash Buffer (approximately 350µL)using a squirt bottle, multi-channel pipette, manifold dispenser orautomated washer. Complete removal of liquid at each step is essentialto good performance. After the last wash, remove any remaining WashBuffer by aspirating or decanting. Invert the plate and pat it againstthick clean absorbent paper. |
| 4. | HRP-Streptavidin Conjugate(SABC): Add 100µL of SABC workingsolution to each well. Cover with a new Plate sealer. Incubate for30minutes at 37°C. |
| 5. | Wash: Repeat the aspiration/wash process for five times. |
| 6. | TMB Substrate: Add 90µL of TMB Substrate to each well. Coverwith a new Plate sealer. Incubate for about 10-20 minutes at 37°C.Protect from light. The reaction time can be shortened or extendedaccording to the actual color change, but not more than 30minutes.When apparent gradient appeared in standard wells, you can terminatethe reaction. |
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