Description
| Product Name: | Mouse CNTF Recombinant Protein |
| Product Code: | RPPB0130 |
| Size: | 25µg |
| Species: | Mouse |
| Target: | CNTF |
| Synonyms: | HCNTF, CNTF, Ciliary Neurotrophic Factor. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2�m filtered concentrated solution in PBS, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized CNTF in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Ciliary Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CNTF should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | MAFAEQSPLT LHRRDLCSRS IWLARKIRSD LTALMESYVK HQGLNKNISL DSVDGVPVAS TDRWSEMTEA ERLQENLQAY RTFQGMLTKL LEDQRVHFTP TEGDFHQAIH TLTLQVSAFA YQLEELMALL EQKVPEKEAD GMPVTIGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRVIS SHHMGISAHE SHYGAKQM |
| Biological Activity: | Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of TF-1 cells is less than 35ng/ml, corresponding to a Specific Activity of 3.0�104 IU/mg. |
CNTF is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. In addition to the predominant monocistronic transcript originating from this locus, the gene is also co-transcribed with the upstream ZFP91 gene. Co-transcription from the two loci results in a transcript that contains a complete coding region for the zinc finger protein but lacks a complete coding region for ciliary neurotrophic factor.CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
Ciliary Neurotrophic Factor Recombinant Mouse produced in E.Coli is a single, non-glycosylated polypeptide chain containing 198 amino acids and having a molecular mass of 22.6kDa. The CNTF is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CNTF: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. Belongs to the CNTF family. |
| UniProt Protein Details: | Cellular Component: extracellular space; cell soma; axon; cytoplasm; nucleus; cytosol Molecular Function:interleukin-6 receptor binding; growth factor activity; cytokine activity; ciliary neurotrophic factor receptor binding Biological Process: negative regulation of photoreceptor cell differentiation; nervous system development; positive regulation of axon regeneration; protein heterooligomerization; multicellular organismal development; membrane hyperpolarization; positive regulation of hormone secretion; positive regulation of JAK-STAT cascade; JAK-STAT cascade; positive regulation of cell growth; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of MAPKKK cascade; regulation of resting membrane potential; positive regulation of cell proliferation; neuron development; regulation of retinal cell programmed cell death; negative regulation of neuron apoptosis; cell differentiation; astrocyte activation; muscle morphogenesis; growth |
| NCBI Summary: | The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes, and it may be involved in reducing tissue destruction during inflammatory attacks. A read-through transcript variant composed of Zfp91 and Cntf sequences has been identified, but it is thought to be non-coding. Read-through transcription of Zfp91 and Cntf has been observed in both human and mouse. [provided by RefSeq, Aug 2008] |
| UniProt Code: | P51642 |
| NCBI GenInfo Identifier: | 1705963 |
| NCBI Gene ID: | 12803 |
| NCBI Accession: | P51642.1 |
| UniProt Related Accession: | P51642 |
| Molecular Weight: | 22,587 Da |
| NCBI Full Name: | Ciliary neurotrophic factor |
| NCBI Synonym Full Names: | ciliary neurotrophic factor |
| NCBI Official Symbol: | Cntf�� |
| NCBI Official Synonym Symbols: | AI429687�� |
| NCBI Protein Information: | ciliary neurotrophic factor |
| UniProt Protein Name: | Ciliary neurotrophic factor |
| Protein Family: | Ciliary neurotrophic factor |
| UniProt Gene Name: | Cntf�� |
| UniProt Entry Name: | CNTF_MOUSE |
