Mouse Chemokine Array (25 targets) (SARB0076)

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SKU:
SARB0076

Description

system_update_altDatasheet
Product Sku:SARB0076
Size:2, 4, or 8
Species Detected:Mouse
Number of Targets Detected:25
Gene Symbols:CCL1, CCL11, CCL12, CCL17, CCL19, CCL2, CCL20, CCL21, CCL22, CCL24|CCL25, CCL27, CCL3, CCL5, CCL9, CX3CL1, CXCL1, CXCL11, CXCL12, CXCL13, CXCL16, CXCL2, CXCL5, CXCL9, PF4
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
6Ckine (CCL21)
  • Gene Symbol: CCL21
  • Gene ID: N/A
  • Accession #: P86792
BLC (CXCL13)
  • Gene Symbol: CXCL13
  • Gene ID: N/A
  • Accession #: O55038
CTACK (CCL27)
  • Gene Symbol: CCL27
  • Gene ID: N/A
  • Accession #: Q9Z1X0
CXCL16
  • Gene Symbol: CXCL16
  • Gene ID: N/A
  • Accession #: Q5F2D5
Eotaxin-1 (CCL11)
  • Gene Symbol: CCL11
  • Gene ID: N/A
  • Accession #: P48298
Eotaxin-2 (MPIF-2/CCL24)
  • Gene Symbol: Ccl24
  • Gene ID: N/A
  • Accession #: Q9JKC0
Fractalkine (CX3CL1)
  • Gene Symbol: CX3CL1
  • Gene ID: N/A
  • Accession #: O35188
I-TAC (CXCL11)
  • Gene Symbol: CXCL11
  • Gene ID: N/A
  • Accession #: Q9JHH5
KC (CXCL1)
  • Gene Symbol: CXCL1
  • Gene ID: N/A
  • Accession #: P12850
LIX
  • Gene Symbol: Cxcl5
  • Gene ID: N/A
  • Accession #: P50228
MCP-1 (CCL2)
  • Gene Symbol: CCL2
  • Gene ID: N/A
  • Accession #: Q9QYD7
MCP-5
  • Gene Symbol: Ccl12
  • Gene ID: N/A
  • Accession #: Q9QYD6
MDC (CCL22)
  • Gene Symbol: CCL22
  • Gene ID: N/A
  • Accession #: O88430
MIG (CXCL9)
  • Gene Symbol: CXCL9
  • Gene ID: N/A
  • Accession #: P18340
MIP-1 alpha (CCL3)
  • Gene Symbol: CCL3
  • Gene ID: N/A
  • Accession #: P14096
MIP-1 gamma
  • Gene Symbol: Ccl9
  • Gene ID: N/A
  • Accession #: Q5QNW2
MIP-2
  • Gene Symbol: Cxcl2
  • Gene ID: N/A
  • Accession #: P10889
MIP-3 beta (CCL19)
  • Gene Symbol: CCL19
  • Gene ID: N/A
  • Accession #: O70460
MIP-3 alpha (CCL20)
  • Gene Symbol: CCL20
  • Gene ID: N/A
  • Accession #: O89093
Platelet Factor 4 (CXCL4)
  • Gene Symbol: PF4, CXCL4, SCYB4
  • Gene ID: N/A
  • Accession #: Q9Z126
RANTES (CCL5)
  • Gene Symbol: CCL5
  • Gene ID: N/A
  • Accession #: P30882
SDF-1 (CXCL12)
  • Gene Symbol: CXCL12
  • Gene ID: N/A
  • Accession #: P40224
TARC (CCL17)
  • Gene Symbol: CCL17
  • Gene ID: N/A
  • Accession #: Q9WUZ6
I-309 (TCA-3/CCL1)
  • Gene Symbol: CCL1
  • Gene ID: N/A
  • Accession #: P14098
TECK (CCL25)
  • Gene Symbol: CCL25
  • Gene ID: N/A
  • Accession #: O35903
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Mouse Chemokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product Type:
Protein Array
Analytes:
6Ckine (CCL21)
Analytes:
BLC (CXCL13)
Analytes:
CTACK (CCL27)
Analytes:
CXCL16
Analytes:
Eotaxin-1 (CCL11)
Analytes:
Eotaxin-2 (MPIF-2/CCL24)
Analytes:
Fractalkine (CX3CL1)
Analytes:
I-TAC (CXCL11)
Analytes:
KC (CXCL1)
Analytes:
LIX
Analytes:
MCP-1 (CCL2)
Analytes:
MCP-5
Analytes:
MDC (CCL22)
Analytes:
MIG (CXCL9)
Analytes:
MIP-1 alpha (CCL3)
Analytes:
MIP-1 gamma
Analytes:
MIP-2
Analytes:
MIP-3 beta (CCL19)
Analytes:
MIP-3 alpha (CCL20)
Analytes:
Platelet Factor 4 (CXCL4)
Analytes:
RANTES (CCL5)
Analytes:
SDF-1 (CXCL12)
Analytes:
TARC (CCL17)
Analytes:
I-309 (TCA-3/CCL1)
Analytes:
TECK (CCL25)
Reactivity:
Mouse
Applications:
Multiplex Array
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