Enzymes Recombinant Proteins

Mouse CES1G Recombinant Protein (RPPB1486)

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SKU:
RPPB1486
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
Q8VCC2
Research Area:
Enzymes

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Mouse CES1G Recombinant Protein
Product Code:RPPB1486
Size:10µg
Species:Mouse
Target:CES1G
Synonyms:Liver carboxylesterase 1, Acyl-coenzyme A:cholesterol acyltransferase, Carboxylesterase 1G, ES-x, CES1G.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered colorless solution.
Formulation:CES1G protein solution (0.5mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles.
Purity:Greaterthan 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:ADPHPSLPPVVHTVHGKVLG KYVTLEGFSQ PVAVFLGVPF AKPPLGSLRF APPEPAEPWS FVKHTTSYPP LCYQNPEAALRLAELFTNQR KIIPHKFSED CLYLNIYTPA DLTQNSRLPV MVWIHGGGLV IDGASTYDGV PLAVHENVVVVVIQYRLGIW GFFSTEDEHS RGNWGHLDQV AALHWVQDNI ANFGGNPGSV TIFGESAGGE SVSVLVLSPLAKNLFHRAIA QSSVIFNPCL FGRAARPLAK KIAALAGCKT TTSAAMVHCL RQKTEDELLE VSLKMKFGTVDFLGDPRESY PFLPTVIDGV LLPKAPEEIL AEKSFNTVPY MVGINKHEFG WIIPMFLDFP LSERKLDQKT AASILWQAYPILNISEKLIP AAIEKYLGGT EDPATMTDLF LDLIGDIMFG VPSVIVSRSH RDAGAPTYMY EYQYRPSFVSDDRPQELLGD HADELFSVWG APFLKEGASE EEINLSKMVM KFWANFARNG NPNGEGLPHW PEYDQKEGYLQIGVPAQAAH RLKDKEVDFW TELRAKETAE RSSHREHVEL HHHHHH
Biological Activity:Specific activity is > 2,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH 7.5 at 37C.
UniProt Code:Q8VCC2

Carboxylesterase1 (CES1G) belongs to a large family of carboxylesterases that are liable forthe hydrolysis of ester and amide bonds. CES1G is also participates in thedetoxification of xenobiotics prodrugs. CES1G shares the serinehydrolase fold observed in other esterases. CES1G found in rats and mice and isexpressed mainly in liver, but also in kidney and lung.

CES1G produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 556 amino acids (19-565 a.a.) and having a molecular mass of 61.9kDa (Migrates at 50-70kDa on SDS-PAGE under reducing conditions). CES1G is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

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