Description
| Product Name: | Mouse CDH5 Recombinant Protein |
| Product Code: | RPPB3129 |
| Size: | 5µg |
| Species: | Mouse |
| Target: | CDH5 |
| Synonyms: | Cadherin-5,Vascular endothelial cadherin, VE-cadherin, CD144, Cdh5. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterilefiltered colorless solution. |
| Formulation: | CDH5 protein solution (0.5mg/ml) contains phosphate buffered saline (pH7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greaterthan 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | GPNFPQIDTP NMLPAHHRQK RDWIWNQMHI DEEKNESLPHYVGKIKSNVN RQNAKYVLQG EFAGKIFGVD ANTGNVLAYE RLDREKVSEY FLTALIVDKN TNKNLEQPSSFTVKVHDIND NWPVFSHQVF NASVPEMSAI GTSVIRVTAV DADDPTVAGH ATVLYQIVKG NEYFSIDNSGLIFTKIKNLD REKQAEYKIV VETQDALGLR GESGTATVMI RLEDINDNFP VFTQSTYTFS VPEDIRVGKPLGFLTVVDPD EPQNRMTKYS IMQGEYRDTF TIETDPKRNE GIIKPTKSLD YEVIQQYTFY IEATDPTIRYEYLSSTSGKN KAMVTINVLD VDEPPVFQRH FYHFKLPENQ KKPLIGTVVA KDPDKAQRSI GYSIRKTSDRGQFFRITKQG NIYNEKELDR ETYAWYNLTV EANELDSRGN PVGKESIVQV YIEVLDENDN PPEFAQPYEPKVCENAAQGK LVVQISATDK DVVPVNPKFK FALKNEDSNF TLINNHDNTA NITVKYGQFN REHAKFHYLPVLISDNGVPS LTGTSTLTVG VCKCNEQGEF TFCEEMAAQA GVSIQLEHHH HHH |
Cadherin-5, also known as CDH5 is a classical cadherin which belongs tothe cadherin superfamily. The CDH5 is located in a six-cadherincluster in a region on the long arm of chromosome 16 which is involved in lossof heterozygosity events in breast as well as prostate cancer.CDH5 is a calcium-dependent cell-cell adhesion glycoproteincomposed of five extracellular cadherin repeats, a transmembrane region and ahighly conserved cytoplasmic tail. Furthermore, CDH5 functionsas a classic cadherin by passing on to cells the ability to adhere in a homophilic manner, CDH5�plays a significant role in endothelial cell biology through control ofthe cohesion as well as organization of the intercellular junctions.
CDH5 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 583 amino acids (25-599a.a.) and having a molecular mass of 66.2kDa. (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).CDH5 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral Cellular Component: adherens junction; cell junction; cell surface; external side of plasma membrane; integral to membrane; intercellular junction; membrane; plasma membrane; tight junction Molecular Function:beta-catenin binding; calcium ion binding; metal ion binding; protein binding; protein phosphatase binding; receptor binding Biological Process: blood vessel maturation; cell adhesion; cell-cell adhesion; homophilic cell adhesion; intercellular junction assembly; negative regulation of cell proliferation; transforming growth factor beta receptor signaling pathway |
| NCBI Summary: | This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. Mice lacking the encoded protein die in utero due to vascular insufficiency, caused by increased endothelial apoptosis. Multiple distinct genes of the cadherin family, including this gene, are found on chromosome 8. [provided by RefSeq, Oct 2015] |
| UniProt Code: | P55284 |
| NCBI GenInfo Identifier: | 34588132 |
| NCBI Gene ID: | 12562 |
| NCBI Accession: | P55284.2 |
| UniProt Secondary Accession: | P55284,O35542, |
| UniProt Related Accession: | P55284 |
| Molecular Weight: | 87,903 Da |
| NCBI Full Name: | Cadherin-5 |
| NCBI Synonym Full Names: | cadherin 5 |
| NCBI Official Symbol: | Cdh5�� |
| NCBI Official Synonym Symbols: | 7B4; Vec; VECD; Cd144; VEcad; VE-Cad; AA408225�� |
| NCBI Protein Information: | cadherin-5 |
| UniProt Protein Name: | Cadherin-5 |
| UniProt Synonym Protein Names: | Vascular endothelial cadherin; VE-cadherin; CD_antigen: CD144 |
| Protein Family: | Cadherin |
| UniProt Gene Name: | Cdh5�� |
| UniProt Entry Name: | CADH5_MOUSE |
