Description
Antibody Name: | Mouse CD90.2 Monoclonal Antibody (PE/Cyanine5 Conjugated) |
Antibody Code: | AGEL1216 |
Size: | 50µg |
Clone: | 30H12 |
Synonyms: | Thy-1.2 membrane glycoprotein,Thy1.2,Thy-1.2 antigen,CD90.2,Thy-1.2 |
Host Species: | Rat |
Species Reactivity: | Mouse |
Applications: | FC |
Concentration: | 1mg/mL |
Isotype: | IgG2a,k |
Conjugation: | PE/Cyanine5 |
Applications: | FC |
Recommended Dilution: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10e6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Storage Buffer: | PBS with 0.05% Proclin300, 1% BSA |
Storage: | Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. |
Gene ID: | 21838 |
Uniprot: | P01831 |
Background: | CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30H12 antibody has been reported to induce Ca2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes. |
C57BL/6 murine splenocytes are stained with Anti-Mouse CD90.2 Monoclonal Antibody(PE/Cyanine5 Conjugated)[Used at ?g/106 cells dilution](filled gray histogram). Unstained splenocytes (empty black histogram) are used as control. |