Mouse CD62L Monoclonal Antibody (PerCP/Cyanine5.5 Conjugated) [Mel14](AGEL0632)

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SKU:
AGEL0632
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Applications:
FC
Reactivity:
Mouse
Host Species:
Rat
Isotype:
IgG2a,k
Clone:
Mel14
Conjugation:
PerCP/Cy5.5

Description

Antibody Name:Mouse CD62L Monoclonal Antibody (PerCP/Cyanine5.5 Conjugated)
Antibody Code:AGEL0632
Size:50 Tests
Clone:Mel14
Synonyms:L-selectin,Sell,CD62 antigen-like family member L,LAM-1,LECAM1,Lymph node homing receptor,Ly-22, CD62L,Lnhr,Ly22
Host Species:Rat
Species Reactivity:Mouse
Applications:FC
Concentration:5uL
Isotype:IgG2a,k
Conjugation:PerCP/Cyanine5.5
Applications:FC
Recommended Dilution:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 uL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage Buffer:PBS with 0.05% Proclin300, 1% BSA
Storage:Store at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Gene ID:20343
Uniprot:P18337
Background:CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.
Mouse CD19 Monoclonal Antibody (FITC Conjugated)C57BL/6 murine splenocytes are stained with Anti-Mouse CD62L Monoclonal Antibody(PerCP/Cyanine5.5 Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.
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