CD Antigens Recombinant Proteins

Mouse CD55 Recombinant Protein (RPPB3080)

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RPPB3080

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Mouse CD55 Recombinant Protein
Product Code:RPPB3080
Size:10µg
Species:Mouse
Target:CD55
Synonyms:Complement decay-accelerating factor, GPI-anchored,DAF-GPI, CD_antigen: CD55.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered colorless solution.
Formulation:CD55protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10%glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles.
Purity:Greaterthan 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:DCGPPPDIPN ARPILGRHSK FAEQSKVAYS CNNGFKQVPD KSNIVVCLENGQWSSHETFC EKSCVAPERL SFASLKKEYL NMNFFPVGTI VEYECRPGFR KQPPLPGKAT CLEDLVWSPVAQFCKKKSCP NPKDLDNGHI NIPTGILFGS EINFSCNPGY RLVGVSSTFC SVTGNTVDWD DEFPVCTEIHCPEPPKINNG IMRGESDSYT YSQVVTYSCD KGFILVGNAS IYCTVSKSDV GQWSSPPPRC IEKSKVPTKKPTINVPSTGT PSTPQKPTTE SVPNPGDQPT PQKPSTVKVS ATQHVPVTKT TVRHPIRTST DKGEPNTGLEHHHHHH

CD55belongs to the RCA (regulators of complement activation) family characterizedby 4-30 SCRs (short consensus repeats) in their plasma-exposed regions. CD55 iswidely expressed in malignant tumors and nevertheless functions as an inhibitorof the complement system.

CD55 produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 336 amino acids (35-362 a.a.) andhaving a molecular mass of 36.8kDa (Migrates at 40-57kDa on SDS-PAGE underreducing conditions).CD55 is expressed with an 8 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:CD55: This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Belongs to the receptors of complement activation (RCA) family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Cell surface; Membrane protein, GPI anchorChromosomal Location of Human Ortholog: 1 E4|1 56.89 cMCellular Component: apical plasma membrane; cell surface; external side of plasma membrane; membrane; membrane raft; plasma membraneMolecular Function: enzyme inhibitor activity; lipid binding; viral receptor activityBiological Process: complement activation, classical pathway; immune system process; innate immune response; regulation of complement activation, classical pathway
UniProt Protein Details:
NCBI Summary:This gene encodes an inhibitor of both the classical and the alternative pathways of complement activation. The encoded preproprotein undergoes post-translational processing to generate a mature polypeptide anchored to the plasma membrane via a glycosylphosphatidylinositol moiety. Erythrocytes from mice deficient in the encoded protein exhibit impaired regulation of complement activation resulting in enhanced complement deposition. Mice lacking the encoded protein exhibit enhanced susceptibility to experimentally induced myasthenia gravis. This gene is located adjacent to a closely related gene on chromosome 1. [provided by RefSeq, Nov 2015]
UniProt Code:Q61475
NCBI GenInfo Identifier:114326522
NCBI Gene ID:13136
NCBI Accession:NP_034146.2
UniProt Secondary Accession:Q61475,P97732, Q3TU32, Q4FJS4, Q61397, Q76N72, Q921P0 Q9R1C1
UniProt Related Accession:Q61475
Molecular Weight:42,618 Da
NCBI Full Name:complement decay-accelerating factor, GPI-anchored preproprotein
NCBI Synonym Full Names:CD55 molecule, decay accelerating factor for complement
NCBI Official Symbol:Cd55��
NCBI Official Synonym Symbols:Daf; Daf1; Daf-GPI; GPI-DAF��
NCBI Protein Information:complement decay-accelerating factor, GPI-anchored
UniProt Protein Name:Complement decay-accelerating factor, GPI-anchored
UniProt Synonym Protein Names:CD_antigen: CD55
Protein Family:
UniProt Gene Name:Cd55��
UniProt Entry Name:
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Additional Information

Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
Q61475
Research Area:
CD Antigens
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system_update_altDatasheetHuman CD55/DAF Recombinant Protein CD55 is a member of the RCA (regulators of complement activation) family. RCA proteins is characterized by the presence of four to 30