Description
system_update_altDatasheet
Antibody Name: | Mouse CD54 Monoclonal Antibody (Biotin Conjugated) |
Antibody Code: | AGEL0117 |
Size: | 100µg |
Clone: | YN1/1.7.4 |
Synonyms: | Intercellular adhesion molecule 1, Icam1, MALA-2, MyD10, CD54, Icam-1 |
Host Species: | Rat |
Species Reactivity: | Mouse |
Applications: | FC |
Concentration: | 1mg/mL |
Isotype: | IgG2b |
Conjugation: | Biotin |
Applications: | FC |
Recommended Dilution: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10e6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Storage Buffer: | PBS with 0.05% Proclin300, 1% BSA |
Storage: | Keep as concentrated solution.Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Gene ID: | 15894 |
Uniprot: | P13597 |
Background: | CD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues. |