Description
system_update_altDatasheet
| Product Name: | Mouse CD3D & CD3E Recombinant Protein (RPES2183) |
| Product Code: | RPES2183 |
| Size: | 20µg |
| Species: | Mouse |
| Expressed Host: | HEK293 Cells |
| Synonyms: | T3d |
| Accession: | P04235&P22646 |
| Sequence: | Met 1-Ala 105&Met 1-Asp 108 |
| Fusion tag: | C-Flag-Fc, C-His-Fc |
| Endotoxin: | <1.0 EU per µg of the protein as determined by the LAL method. |
| Protein Construction: | A DNA sequence encoding the extracellular domain (Met 1-Ala 105) of mouse CD3D (P04235) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 108) of mouse CD3E (P22646) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified. |
| Purity: | > 90 % as determined by SDS-PAGE |
| Mol Mass: | 76 kDa |
| AP Mol Mass: | 45-50 kDa |
| Formulation: | Lyophilized from sterile PBS, pH 7.4 |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
