Description
Antibody Name: | Mouse CD3 Monoclonal Antibody (PE/Cyanine5 Conjugated) |
Antibody Code: | AGEL0659 |
Size: | 50µg |
Clone: | 17A2 |
Synonyms: | T-cell surface glycoprotein CD 3epsilon/delta/gamma/zeta chain,CD3E/D/G/Z,CD3e/d/g/z,CD3E/D/G/Z,CD3 |
Host Species: | Rat |
Species Reactivity: | Mouse |
Applications: | FC |
Concentration: | 1mg/mL |
Isotype: | IgG2a,k |
Conjugation: | PE/Cyanine5 |
Applications: | FC |
Recommended Dilution: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10e6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Storage Buffer: | PBS with 0.05% Proclin300, 1% BSA |
Storage: | Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. |
Gene ID: | 12502 |
Uniprot: | P04235, P11942, P22646, P24161, P29020 |
Background: | CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. |
C57BL/6 murine splenocytes are stained with Anti-Mouse CD3 Monoclonal Antibody(PE/Cyanine5 Conjugated)[Used at 0.02 ?g/106 cells dilution](filled gray histogram). Unstained splenocytes (empty black histogram) are used as control. |