Description
system_update_altDatasheet
Antibody Name: | Mouse CD3 Monoclonal Antibody (APC Conjugated) |
Antibody Code: | AGEL0100 |
Size: | 200 Tests |
Clone: | 17A2 |
Synonyms: | T-cell surface glycoprotein CD 3epsilon/delta/gamma/zeta chain, CD3E/D/G/Z, CD3e/d/g/z, CD3E/D/G/Z, CD3 |
Host Species: | Rat |
Species Reactivity: | Mouse |
Applications: | FC |
Concentration: | 5uL |
Isotype: | IgG2b |
Conjugation: | APC |
Applications: | FC |
Recommended Dilution: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 uL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Storage Buffer: | PBS with 0.05% Proclin300, 1% BSA |
Storage: | Keep as concentrated solution.Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Gene ID: | 12502 |
Uniprot: | P04235, P11942, P22646, P24161, P29020 |
Background: | CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. |
C57BL/6 murine splenocytes are stained with Anti-Mouse CD3 Monoclonal Antibody(APC Conjugated)(filled gray histogram). Unstained splenocytes(empty black histogram) are used as control. |