](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/1280x1280/products/11930/15984/mouse-cd3-monoclonal-antibody-af647-conjugated-17a2agel0664__35354__62183.1706269759.jpg?c=1&imbypass=on "Mouse CD3 Monoclonal Antibody (AF647 Conjugated) [17A2](AGEL0664)")
Description
| Antibody Name: | Mouse CD3 Monoclonal Antibody (AF647 Conjugated) |
| Antibody Code: | AGEL0664 |
| Size: | 50µg |
| Clone: | 17A2 |
| Synonyms: | T-cell surface glycoprotein CD 3epsilon/delta/gamma/zeta chain,CD3E/D/G/Z,CD3e/d/g/z,CD3E/D/G/Z,CD3 |
| Host Species: | Rat |
| Species Reactivity: | Mouse |
| Applications: | FC |
| Concentration: | 1mg/mL |
| Isotype: | IgG2a,k |
| Conjugation: | AF647 |
| Applications: | FC |
| Recommended Dilution: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10e6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
| Storage Buffer: | PBS with 0.05% Proclin300, 1% BSA |
| Storage: | Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. |
| Gene ID: | 12502 |
| Uniprot: | P04235, P11942, P22646, P24161, P29020 |
| Background: | CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. |
 | C57BL/6 murine splenocytes are stained with Anrti-Mouse CD3 Monoclonal Antibody(AF647 Conjuaged)[Used at 0.2 ?g/106 cells dilution](filled gray histogram). Unstained lymphocytes (empty black histogram) are used as control. |
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