Description
system_update_altDatasheet
Antibody Name: | Mouse CD28 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
Antibody Code: | AGEL0139 |
Size: | 50 Tests |
Clone: | 37.51 |
Synonyms: | T-cell-specific surface glycoprotein CD28, Cd28, CD28 |
Host Species: | Hamster |
Species Reactivity: | Mouse |
Applications: | FC |
Concentration: | 5uL |
Isotype: | IgG |
Conjugation: | Alexa Fluor 488 |
Applications: | FC |
Recommended Dilution: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 uL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Storage Buffer: | PBS with 0.05% Proclin300, 1% BSA |
Storage: | Keep as concentrated solution.Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Gene ID: | 12487 |
Uniprot: | P31041 |
Background: | CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development. |
C57BL/6 murine splenocytes are stained with Anti-Mouse CD28 Monoclonal Antibody (Alexa Fluor 488 Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control. |