Mouse CD28 Monoclonal Antibody (AF647 Conjugated) [37.51](AGEL0774)

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SKU:
AGEL0774
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Applications:
FC
Reactivity:
Mouse
Host Species:
Hamster
Isotype:
IgG2a,k
Clone:
37.51
Conjugation:
AF 647

Description

Antibody Name:Mouse CD28 Monoclonal Antibody (AF647 Conjugated)
Antibody Code:AGEL0774
Size:50µg
Clone:37.51
Synonyms:T-cell-specific surface glycoprotein CD28,Cd28,CD28
Host Species:Hamster
Species Reactivity:Mouse
Applications:FC
Concentration:1mg/mL
Isotype:IgG2a,k
Conjugation:AF647
Applications:FC
Recommended Dilution:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10e6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Storage Buffer:PBS with 0.05% Proclin300, 1% BSA
Storage:Store at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Gene ID:12487
Uniprot:P31041
Background:CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.
Mouse CD19 Monoclonal Antibody (FITC Conjugated)C57BL/6 murine splenocytes are stained with Anti-Mouse CD28 Monoclonal Antibody(AF647 Conjuaged) and Anti-Mouse CD3 Monoclonal Antibody(AF488 Conjugated)[Both antibodies are used at 0.2 ?g/106 cells dilution]. Splenocytes stained with Anti-Mouse CD3 Monoclonal Antibody(AF488 Conjugated)(right) are used as control.
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