](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/1280x1280/products/12575/16627/mouse-cd279pd-1-monoclonal-antibody-pe-conjugated-29f.1a12agel1628__38226__53394.1706270069.jpg?c=1?imbypass=on "Mouse CD279/PD-1 Monoclonal Antibody (PE Conjugated) [29F.1A12](AGEL1628)")
Description
| Antibody Name: | Mouse CD279/PD-1 Monoclonal Antibody (PE Conjugated) |
| Antibody Code: | AGEL1628 |
| Size: | 50 Tests |
| Clone: | 29F.1A12 |
| Synonyms: | PD-1, Programmed Death-1 |
| Host Species: | Rat |
| Species Reactivity: | Mouse |
| Applications: | FC |
| Concentration: | 5uL |
| Isotype: | IgG2a,k |
| Conjugation: | PE |
| Applications: | FC |
| Recommended Dilution: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 uL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
| Storage Buffer: | PBS with 0.05% Proclin300, 1% BSA |
| Storage: | Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. |
| Gene ID: | 18566 |
| Uniprot: | Q02242 |
| Background: | CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts. |
 | Con-A stimulated C57BL/6 splenocytes (3 days) are stained with Anti-Mouse CD279/PD-1 Monoclonal Antibody(PE Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control. |
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