Description
Antibody Name: | Mouse CD25 Monoclonal Antibody (FITC Conjugated) |
Antibody Code: | AGEL1297 |
Size: | 50 Tests |
Clone: | PC-61.5.3 |
Synonyms: | Interleukin-2 receptor subunit alpha,IL2RA,IL-2 receptor subunit alpha,IL-2-RA,IL-2R subunit alpha,IL2-RA,TAC antigen,p55,p55 |
Host Species: | Rat |
Species Reactivity: | Mouse |
Applications: | FC |
Concentration: | 5uL |
Isotype: | IgG2a,k |
Conjugation: | FITC |
Applications: | FC |
Recommended Dilution: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 uL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Storage Buffer: | PBS with 0.05% Proclin300, 1% BSA |
Storage: | Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. |
Gene ID: | 16184 |
Uniprot: | P01590 |
Background: | CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor. |
C57BL/6 murine splenocytes are stained with Anti-Mouse CD25 Monoclonal Antibody(FITC Conjugated) and Anti-Mouse CD4 Monoclonal Antibody(APC Conjugated)(right)). Splenocytes stained with Anti-Mouse CD4 Monoclonal Antibody(APC Conjugated)(left) are used as control. |