Mouse CD25 Monoclonal Antibody (AF647 Conjugated) [PC-61.5.3](AGEL1313)

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SKU:
AGEL1313
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Applications:
FC
Reactivity:
Mouse
Host Species:
Rat
Isotype:
IgG2a,k
Clone:
PC-61.5.3
Conjugation:
AF 647

Description

Antibody Name:Mouse CD25 Monoclonal Antibody (AF647 Conjugated)
Antibody Code:AGEL1313
Size:50µg
Clone:PC-61.5.3
Synonyms:Interleukin-2 receptor subunit alpha,IL2RA,IL-2 receptor subunit alpha,IL-2-RA,IL-2R subunit alpha,IL2-RA,TAC antigen,p55,p55
Host Species:Rat
Species Reactivity:Mouse
Applications:FC
Concentration:1mg/mL
Isotype:IgG2a,k
Conjugation:AF647
Applications:FC
Recommended Dilution:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10e6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Storage Buffer:PBS with 0.05% Proclin300, 1% BSA
Storage:Store at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Gene ID:16184
Uniprot:P01590
Background:CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.
Mouse CD19 Monoclonal Antibody (FITC Conjugated)C57BL/6 murine splenocytes are stained with Anti-Mouse CD25 Monoclonal Antibody(AF647 Conjugated) and Anti-Mouse CD4 Monoclonal Antibody(FITC Conjugated)[Both antibodies are used at 0.2 ?g/106 cells dilution]. Splenocytes stained with Anti-Mouse CD4 Monoclonal Antibody(FITC Conjugated)and Rat IgG1 Isotype Control(AF647 Conjugated)(right) are used as control.
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