Mouse CD162 Monoclonal Antibody [4RA10](AGEL0159)

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SKU:
AGEL0159
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Mouse
Host Species:
Rat
Isotype:
IgG1
Clone:
4RA10
Conjugation:
Unconjugated
Applications:
FC
Applications:
WB
Applications:
Block
Applications:
IP

Description

system_update_altDatasheet
Antibody Name:Mouse CD162 Monoclonal Antibody
Antibody Code:AGEL0159
Size:100µg
Clone:4RA10
Synonyms:P-selectin glycoprotein ligand 1, Selplg, PSGL-1, Selectin P ligand, CD162
Host Species:Rat
Species Reactivity:Mouse
Applications:FC, WB, Block, IP
Concentration:1mg/mL
Isotype:IgG1
Conjugation:Unconjugated
Applications:FC, WB, Block, IP
Recommended Dilution:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10e6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Storage Buffer:PBS with 0.05% Proclin300
Storage:Keep as concentrated solution.Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Gene ID:20345
Uniprot:Q62170
Background:The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.
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