Mouse CD127/IL-7RA Monoclonal Antibody (PerCP/Cyanine5.5 Conjugated) [A7R34](AGEL0743)

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SKU:
AGEL0743
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Applications:
FC
Reactivity:
Mouse
Host Species:
Rat
Isotype:
IgG2a,k
Clone:
A7R34
Conjugation:
PerCP/Cy5.5

Description

Antibody Name:Mouse CD127/IL-7RA Monoclonal Antibody (PerCP/Cyanine5.5 Conjugated)
Antibody Code:AGEL0743
Size:50 Tests
Clone:A7R34
Synonyms:Interleukin-7 receptor subunit alpha,Il7r,IL-7 receptor subunit alpha,IL-7R subunit alpha,IL-7R-alpha,IL-7RA,CD127
Host Species:Rat
Species Reactivity:Mouse
Applications:FC
Concentration:5uL
Isotype:IgG2a,k
Conjugation:PerCP/Cyanine5.5
Applications:FC
Recommended Dilution:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 uL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage Buffer:PBS with 0.05% Proclin300, 1% BSA
Storage:Store at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Gene ID:16197
Uniprot:P16872
Background:CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.
Mouse CD19 Monoclonal Antibody (FITC Conjugated)C57BL/6 murine splenocytes are stained with Anti-Mouse CD127 Monoclonal Antibody(PerCP-Cy5.5 Conjugated) and Anti-Mouse CD3 Monoclonal Antibody(AF488 Conjugated)(left). Splenocytes stained with Anti-Mouse CD3 Monoclonal Antibody(AF488 Conjugated)(right) are used as control.
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