](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/1280x1280/products/11995/16048/mouse-cd127il-7ra-monoclonal-antibody-af647-conjugated-a7r34agel0754__51323__73427.1706269783.jpg?c=1?imbypass=on "Mouse CD127/IL-7RA Monoclonal Antibody (AF647 Conjugated) [A7R34](AGEL0754)")
Description
| Antibody Name: | Mouse CD127/IL-7RA Monoclonal Antibody (AF647 Conjugated) |
| Antibody Code: | AGEL0754 |
| Size: | 50µg |
| Clone: | A7R34 |
| Synonyms: | Interleukin-7 receptor subunit alpha,Il7r,IL-7 receptor subunit alpha,IL-7R subunit alpha,IL-7R-alpha,IL-7RA,CD127 |
| Host Species: | Rat |
| Species Reactivity: | Mouse |
| Applications: | FC |
| Concentration: | 1mg/mL |
| Isotype: | IgG2a,k |
| Conjugation: | AF647 |
| Applications: | FC |
| Recommended Dilution: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10e6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
| Storage Buffer: | PBS with 0.05% Proclin300, 1% BSA |
| Storage: | Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. |
| Gene ID: | 16197 |
| Uniprot: | P16872 |
| Background: | CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development. |
 | C57BL/6 murine splenocytes are stained with Anti-Mouse CD127 Monoclonal Antibody(AF647 Conjuaged) and Anti-Mouse CD3 Monoclonal Antibody(AF488 Conjuaged)[Both antibodies are used at 0.2 ?g/106 cells dilution]. Splenocytes stained with Anti-Mouse CD3 Monoclonal Antibody(AF488 Conjuaged)(right) are used as control. |
](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/608x608/products/11995/16048/mouse-cd127il-7ra-monoclonal-antibody-af647-conjugated-a7r34agel0754__51323__73427.1706269783.jpg?c=1 "Mouse CD127/IL-7RA Monoclonal Antibody (AF647 Conjugated) [A7R34](AGEL0754)")
](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/590x590/products/11989/16042/mouse-cd127il-7ra-monoclonal-antibody-pe-conjugated-a7r34agel0746__66791__86271.1706269782.jpg?c=1 "Mouse CD127/IL-7RA Monoclonal Antibody (PE Conjugated) [A7R34](AGEL0746)")
](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/590x590/products/11994/16047/mouse-cd127il-7ra-monoclonal-antibody-af488-conjugated-a7r34agel0753__08569__80050.1706269783.jpg?c=1 "Mouse CD127/IL-7RA Monoclonal Antibody (AF488 Conjugated) [A7R34](AGEL0753)")
](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/590x590/products/11988/16041/mouse-cd127il-7ra-monoclonal-antibody-fitc-conjugated-a7r34agel0745__93042__86794.1706269781.jpg?c=1 "Mouse CD127/IL-7RA Monoclonal Antibody (FITC Conjugated) [A7R34](AGEL0745)")
](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/590x590/products/11990/16043/mouse-cd127il-7ra-monoclonal-antibody-apc-conjugated-a7r34agel0747__64014__15917.1706269782.jpg?c=1 "Mouse CD127/IL-7RA Monoclonal Antibody (APC Conjugated) [A7R34](AGEL0747)")