Mouse CD122 Monoclonal Antibody (PE/Cyanine5 Conjugated) [5H4](AGEL0802)

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SKU:
AGEL0802
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Applications:
FC
Reactivity:
Mouse
Host Species:
Rat
Isotype:
IgG2a,k
Clone:
5H4
Conjugation:
PE/Cy5

Description

Antibody Name:Mouse CD122 Monoclonal Antibody (PE/Cyanine5 Conjugated)
Antibody Code:AGEL0802
Size:50µg
Clone:5H4
Synonyms:Interleukin-2 receptor subunit beta,Il2rb,IL-2 receptor subunit beta,IL-2R subunit beta,IL-2RB,High affinity IL-2 receptor subunit beta,p70-75,CD122
Host Species:Rat
Species Reactivity:Mouse
Applications:FC
Concentration:1mg/mL
Isotype:IgG2a,k
Conjugation:PE/Cyanine5
Applications:FC
Recommended Dilution:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10e6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Storage Buffer:PBS with 0.05% Proclin300, 1% BSA
Storage:Store at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Gene ID:16185
Uniprot:P16297
Background:CD122 is a 70-75 kD IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The 5H4 antibody does not block IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.
Mouse CD19 Monoclonal Antibody (FITC Conjugated)C57BL/6 murine splenocytes are stained with Anti-Mouse CD122 Monoclonal Antibody(PE/Cyanine5 Conjugated) and Anti-Mouse CD3 Monoclonal Antibody(AF488 Conjugated)[Both antibodies are used at 0.2 ?g/106 cells dilution]. Splenocytes stained with Anti-Mouse CD3 Monoclonal Antibody(AF488 Conjugated)(right) are used as control.
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