](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/1280x1280/products/11057/15111/mouse-cd122-monoclonal-antibody-alexa-fluor-488-conjugated-5h4agel0146__01792__54874.1706269334.jpg?c=1?imbypass=on "Mouse CD122 Monoclonal Antibody (Alexa Fluor 488 Conjugated) [5H4](AGEL0146)")
Description
system_update_altDatasheet
| Antibody Name: | Mouse CD122 Monoclonal Antibody (Alexa Fluor 488 Conjugated) |
| Antibody Code: | AGEL0146 |
| Size: | 50 Tests |
| Clone: | 5H4 |
| Synonyms: | Interleukin-2 receptor subunit beta, Il2rb, IL-2 receptor subunit beta, IL-2R subunit beta, IL-2RB, High affinity IL-2 receptor subunit beta, p70-75, CD122 |
| Host Species: | Rat |
| Species Reactivity: | Mouse |
| Applications: | FC |
| Concentration: | 5uL |
| Isotype: | IgG2a |
| Conjugation: | Alexa Fluor 488 |
| Applications: | FC |
| Recommended Dilution: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 uL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
| Storage Buffer: | PBS with 0.05% Proclin300, 1% BSA |
| Storage: | Keep as concentrated solution.Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
| Gene ID: | 16185 |
| Uniprot: | P16297 |
| Background: | CD122 is a 70-75 kD IL-2 receptor Ī² chain also known as IL-2RĪ², which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2RĪ² chain can combine with either the common Ī³ subunit (Ī³c, CD132) alone or with the Ī³c subunit and the IL-2RĪ± subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The 5H4 antibody does not block IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis. |
 | C57BL/6 murine splenocytes are stained with Anti-Mouse CD122 Monoclonal Antibody (Alexa Fluor 488 Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control. |
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