Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.
Product Name:
Mouse CD112/Nectin-2 Recombinant Protein (RPES2906)
Measured by its binding ability in a functional ELISA.1. Immobilized mouse CD112-His at 10 µg/ml (100 ?l/well) can bind biotinylated mouse DNAM1-His with a linear range of 0.156-5.0 µg/ml.2. Immobilized mouse CD112-His at 10 µg/ml (100 ?l/well) can bind mouse DNAM1-Fc with a linear range of 0.03-1.0 µg/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse PVRL2 (NP_033016.3) extracellular domain (Met 1-Gly 351) was fused with a polyhistidine tag at the C-terminus.
Purity:
> 98 % as determined by SDS-PAGE
Mol Mass:
36 kDa
AP Mol Mass:
40-45 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
system_update_altDatasheetMouse CD112/Nectin-2 Recombinant Protein Nectin-2( CD112) is a member of the nectin family, which contains two Ig-like C2-type domains and one Ig-like V-type domain in t
system_update_altDatasheetRat CD112/Nectin-2 Recombinant Protein Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type
system_update_altDatasheetHuman CD112/Nectin-2 Recombinant Protein CD112 is a type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. It comprises one Ig-like V-type domain
