Mouse Calpain 10 (CAPN10) ELISA Kit

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SKU:
MODL00201
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9ESK3
Sensitivity:
0.103ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
Calcium-activated neutral proteinase 10
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids

Description

system_update_altDatasheet
Product Name:Mouse Calpain 10 (CAPN10) ELISA Kit
Product Code:MODL00201
Size:96 Assays
Detection Method:sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5)82-96%83-98%81-99%93-101%
EDTA plasma (n=5)88-101%86-95%90-102%80-93%
Heparin plasma (n=5)80-91%82-90%95-104%79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.
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