Mouse Developmental Biology ELISA Kits

Mouse Bradykinin ELISA Kit

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SKU:
MOFI00669
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O08677
Sensitivity:
4.688pg/ml
Range:
7.813-500pg/ml
ELISA Type:
Competitive
Synonyms:
BK
Reactivity:
Mouse
Research Area:
Developmental Biology

Description

Mouse Bradykinin ELISA Kit

Bradykinin is a small peptide that plays a crucial role in various physiological processes, including inflammation, vasodilation, pain modulation, and immune responses. It is involved in the regulation of blood flow, vascular permeability, and the release of other inflammatory mediators. The Mouse BK ELISA kit enables quantitative measurement of bradykinin levels specifically in mouse samples, allowing researchers to investigate the role of bradykinin in different physiological and pathological conditions.

system_update_alt Datasheet system_update_alt MSDS

Key Features

Save Time Pre-coated 96 well plate
Quick Start Kit includes all necessary reagents
Publication Ready Reproducible and reliable results

Overview

Product Name:

Mouse Bradykinin ELISA Kit

Product Code:

MOFI00669

Size:

96 Assays

Alias:

BK

Detection Method:

Competitive ELISA

Application:

This immunoassay kit allows for the in vitro quantitative determination of Mouse BK concentrations in serum plasma and other biological fluids.

Sensitivity:

4.688pg/ml

Range:

7.813-500pg/ml

Storage:

4°C for 6 months

Note:

For Research Use Only

Additional Information

Recovery

Matrices listed below were spiked with certain level of Mouse BK and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse BK in samples.

Matrix

Recovery Range (%)

Average (%)

serum (n=5)

88-102

96

EDTA plasma (n=5)

90-105

98

UFH plasma (n=5)

90-101

94

Linearity:

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse ALT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

Serum (n=5)

91-103%

92-104%

89-103%

EDTA plasma (n=5)

86-96%

83-96%

86-97%

UFH Plasma (n=5)

81-98%

81-94%

84-97%

CV(%)

Intra Assay <8

Inter Assay <10

Kit Components

Component Quantity Storage

ELISA Microplate (Dismountable)

8x12 strips

4°C for 6 months

Lyophilized Standard

2

4°C/ -20°C

Sample/Standard Dlution Buffer

20ml

4°C

Biotin-labeled Antibody (Concentrated)

120ul

4°C (Protection from light)

Antibody Dilution Buffer

10ml

4°C

HRP-Streptavidin Conjugate (SABC)

120ul

4°C (Protect from light)

SABC Dilution Buffer

10ml

4°C

TMB Substrate

10ml

4°C (Protection from light)

Stop Solution

10ml

4°C

Wash Buffer (25X)

30ml

4°C

Plate Sealer

5

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