Mouse Cell Signalling ELISA Kits 1
Mouse Bone morphogenetic protein 4 (Bmp4) ELISA Kit
- SKU:
- MOEB0012
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P21275
- Range:
- 15.6-1000 pg/mL
- ELISA Type:
- Sandwich
- Synonyms:
- BMP-4, Bone Morphogenetic Protein 4, BMP2B, BMP2B1, MCOPS6, ZYME, DVR4, OFC11, ZYME
- Reactivity:
- Mouse
Description
| Product Name: | Mouse Bone morphogenetic protein 4 (Bmp4) ELISA Kit |
| Product Code: | MOEB0012 |
| Alias: | Bone morphogenetic protein 4, BMP-4, Bone morphogenetic protein 2B, BMP-2B, Bmp4, Bmp-4, Dvr-4 |
| Uniprot: | P21275 |
| Reactivity: | Mouse |
| Range: | 15.6-1000 pg/mL |
| Detection Method: | Sandwich |
| Size: | 96 Assay |
| Storage: | Please see kit components below for exact storage details |
| Note: | For research use only |
| UniProt Protein Function: | BMP4: Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Homodimer; disulfide-linked. Interacts with GREM2. Part of a complex consisting of TWSG1 and CHRD. Interacts with the serine proteases, HTRA1 and HTRA3; the interaction with either inhibits BMP4-mediated signaling. The HTRA protease activity is required for this inhibition. Interacts with SOSTDC1. Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines. Belongs to the TGF-beta family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Cellular Component: extracellular space; proteinaceous extracellular matrix; cytoplasm; extracellular region Molecular Function:heparin binding; protein binding; protein homodimerization activity; growth factor activity; cytokine activity; transforming growth factor beta receptor binding; chemoattractant activity Biological Process: negative regulation of MAP kinase activity; activation of MAPKK activity; positive regulation of apoptosis; embryonic skeletal development; positive regulation of transcription, DNA-dependent; negative regulation of chondrocyte differentiation; mesodermal cell differentiation; telencephalon regionalization; cardiac muscle cell differentiation; germ cell development; BMP signaling pathway; regulation of protein import into nucleus; anatomical structure formation; kidney development; regulation of odontogenesis of dentine-containing teeth; endochondral ossification; embryonic limb morphogenesis; positive regulation of cardiac muscle fiber development; negative regulation of immature T cell proliferation in the thymus; cell fate commitment; camera-type eye development; regulation of smooth muscle cell proliferation; neuron fate commitment; camera-type eye morphogenesis; regulation of gene expression; negative regulation of mitosis; positive regulation of epidermal cell differentiation; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; negative regulation of apoptosis; tissue development; positive regulation of protein binding; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; inner ear receptor cell differentiation; ureteric bud development; intermediate mesodermal cell differentiation; forebrain development; positive regulation of cell proliferation; angiogenesis; embryonic morphogenesis; positive regulation of BMP signaling pathway; common-partner SMAD protein phosphorylation; negative regulation of T cell differentiation in the thymus; positive regulation of bone mineralization; positive regulation of ossification; odontogenesis of dentine-containing teeth; embryonic skeletal morphogenesis; osteoblast differentiation; positive regulation of osteoblast differentiation; blood vessel endothelial cell proliferation during sprouting angiogenesis; telencephalon development; ureteric bud branching; regulation of cell fate commitment; positive regulation of neuron differentiation; lung development; anterior/posterior axis specification; renal system process; macrophage differentiation; multicellular organismal development; heart development; lymphoid progenitor cell differentiation; post-embryonic development; positive regulation of endothelial cell differentiation; positive chemotaxis; induction of an organ; chondrocyte differentiation; erythrocyte differentiation; specification of organ position; regulation of endothelial cell proliferation; monocyte differentiation; embryonic cranial skeleton morphogenesis; negative regulation of striated muscle development; mesoderm formation; branching morphogenesis of a tube; negative regulation of phosphorylation; steroid hormone mediated signaling; hemopoietic progenitor cell differentiation; positive regulation of endothelial cell proliferation; negative regulation of transcription, DNA-dependent; positive regulation of cell differentiation; metanephros development; alveolus development; positive regulation of epithelial cell proliferation; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; embryonic hindlimb morphogenesis; positive regulation of vascular endothelial growth factor receptor signaling pathway; negative regulation of cell cycle; odontogenesis; smooth muscle development; ovarian follicle development; vasculature development; regulation of cell differentiation; regulation of smooth muscle cell differentiation; cell differentiation; skeletal development; dorsoventral neural tube patterning; negative regulation of epithelial cell proliferation; blood vessel development; ossification; negative regulation of oligodendrocyte differentiation; eye development; pituitary gland development; cartilage development; neural tube closure; positive regulation of protein amino acid phosphorylation; negative regulation of myoblast differentiation; mesodermal cell fate determination; growth |
| UniProt Code: | P21275 |
| NCBI GenInfo Identifier: | 461633 |
| NCBI Gene ID: | 12159 |
| NCBI Accession: | P21275.2 |
| UniProt Related Accession: | P21275 |
| Molecular Weight: | |
| NCBI Full Name: | Bone morphogenetic protein 4 |
| NCBI Synonym Full Names: | bone morphogenetic protein 4 |
| NCBI Official Symbol: | Bmp4 |
| NCBI Official Synonym Symbols: | Bmp-4; Bmp2b; Bmp2b1; Bmp2b-1 |
| NCBI Protein Information: | bone morphogenetic protein 4; bone morphogenetic protein 2B |
| UniProt Protein Name: | Bone morphogenetic protein 4 |
| UniProt Synonym Protein Names: | Bone morphogenetic protein 2B; BMP-2B |
| UniProt Gene Name: | Bmp4 |
| UniProt Entry Name: | BMP4_MOUSE |
| Component | Quantity (96 Assays) | Storage |
| ELISA Microplate (Dismountable) | 8×12 strips | -20°C |
| Lyophilized Standard | 2 | -20°C |
| Sample Diluent | 20ml | -20°C |
| Assay Diluent A | 10mL | -20°C |
| Assay Diluent B | 10mL | -20°C |
| Detection Reagent A | 120µL | -20°C |
| Detection Reagent B | 120µL | -20°C |
| Wash Buffer | 30mL | 4°C |
| Substrate | 10mL | 4°C |
| Stop Solution | 10mL | 4°C |
| Plate Sealer | 5 | - |
Other materials and equipment required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Allow all reagents to reach room temperature (Please do not dissolve the reagents at 37°C directly). All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. Keep appropriate numbers of strips for 1 experiment and remove extra strips from microtiter plate. Removed strips should be resealed and stored at -20°C until the kits expiry date. Prepare all reagents, working standards and samples as directed in the previous sections. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their experiments. We recommend running all samples in duplicate.
| Step | |
| 1. | Add Sample: Add 100µL of Standard, Blank, or Sample per well. The blank well is added with Sample diluent. Solutions are added to the bottom of micro ELISA plate well, avoid inside wall touching and foaming as possible. Mix it gently. Cover the plate with sealer we provided. Incubate for 120 minutes at 37°C. |
| 2. | Remove the liquid from each well, don't wash. Add 100µL of Detection Reagent A working solution to each well. Cover with the Plate sealer. Gently tap the plate to ensure thorough mixing. Incubate for 1 hour at 37°C. Note: if Detection Reagent A appears cloudy warm to room temperature until solution is uniform. |
| 3. | Aspirate each well and wash, repeating the process three times. Wash by filling each well with Wash Buffer (approximately 400µL) (a squirt bottle, multi-channel pipette,manifold dispenser or automated washer are needed). Complete removal of liquid at each step is essential. After the last wash, completely remove remaining Wash Buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper. |
| 4. | Add 100µL of Detection Reagent B working solution to each well. Cover with the Plate sealer. Incubate for 60 minutes at 37°C. |
| 5. | Repeat the wash process for five times as conducted in step 3. |
| 6. | Add 90µL of Substrate Solution to each well. Cover with a new Plate sealer and incubate for 10-20 minutes at 37°C. Protect the plate from light. The reaction time can be shortened or extended according to the actual color change, but this should not exceed more than 30 minutes. When apparent gradient appears in standard wells, user should terminatethe reaction. |
| 7. | Add 50µL of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing. |
| 8. | Determine the optical density (OD value) of each well at once, using a micro-plate reader set to 450 nm. User should open the micro-plate reader in advance, preheat the instrument, and set the testing parameters. |
| 9. | After experiment, store all reagents according to the specified storage temperature respectively until their expiry. |
When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.
| Sample Type | Protocol |
| Serum | If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. |
| Plasma | Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit. |
| Urine & Cerebrospinal Fluid | Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid. |
| Cell culture supernatant | Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately. |
| Cell lysates | Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Tissue homogenates | The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C. |
| Tissue lysates | Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Breast Milk | Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles. |
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