Chemokines Recombinant Proteins

Mouse BCA-1 Recombinant Protein (RPPB1100)

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RPPB1100

Description

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Product Name:Mouse BCA-1 Recombinant Protein
Product Code:RPPB1100
Size:20µg
Species:Mouse
Target:BCA-1
Synonyms:C-X-C motif chemokine 13, B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13, Cxcl13, Blc, Scyb13.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The BCA1 protein was lyophilized from a 0.2�m filtered solution in Acetonitrile and TFA.
Solubility:It is recommended to reconstitute the lyophilized CXCL13 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized BCA1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BCA1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 97.0% as determined by SDS-PAGE.
Amino Acid Sequence:ILEAHYTNLK CRCSGVISTV VGLNIIDRIQ VTPPGNGCPK TEVVIWTKMK KVICVNPRAK WLQRLLRHVQ SKSLSSTPQA PVSKRRAA
Biological Activity:The ED50, as measured by its ability to chemoattract human CXCR5-transfected mouse BaF3 cells, is less than 2�g/ml.

BCA-1 is a CXC chemokine that is highly expressed in thesecondary lymphoid organs, such as follicles of the spleen, lymph nodes, and Peyer's patches. CXCL13 promotes the migration of B lymphocytes (compared to T cells and macrophages), by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR1). BCA1 therefore function in the homing of B lymphocytes to follicles. Human BCA-1 shares a 64% amino acid sequence similarity with the mouse protein and 23 - 34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt�s lymphoma cells, has now been shown to be the specific receptor for BCA1. Among cells of the hematopoietic lineages, the expression of BLR-1, now designated CXCR-5, is restricted to B lymphocytes and a subpopulation of T helper memory cells.

CXCL13 Mouse Recombinant (22-109) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 88 amino acids and having a molecular mass of 10kDa.The BCA-1 is purified by proprietary chromatographic techniques.

UniProt Protein Function:CXCL13: Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B- lymphocytes. Binds to BLR1/CXCR5. Belongs to the intercrine alpha (chemokine CxC) family.
UniProt Protein Details:

Protein type:Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular space; cell; extracellular region; cytosol

Molecular Function:heparin binding; fibroblast growth factor binding; CXCR3 chemokine receptor binding; chemokine activity; protein heterodimerization activity; CXCR5 chemokine receptor binding; CCR10 chemokine receptor binding; cytokine activity; receptor agonist activity

Biological Process: lymphocyte chemotaxis across high endothelial venule; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; chemotaxis; positive regulation of cell-cell adhesion mediated by integrin; positive regulation of integrin activation; lymph node development; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; cell-cell signaling; defense response to bacterium; immune response; inflammatory response

UniProt Code:O55038
NCBI GenInfo Identifier:7673946
NCBI Gene ID:55985
NCBI Accession:O55038.1
UniProt Related Accession:O55038
Molecular Weight:
NCBI Full Name:C-X-C motif chemokine 13
NCBI Synonym Full Names:chemokine (C-X-C motif) ligand 13
NCBI Official Symbol:Cxcl13��
NCBI Official Synonym Symbols:BLC; Angie; BCA-1; BLR1L; ANGIE2; Scyb13; 4631412M08Rik��
NCBI Protein Information:C-X-C motif chemokine 13; CXC chemokine BLC; B lymphocyte chemoattractant; small-inducible cytokine B13; small inducible cytokine subfamily B (Cys-X-Cys), member 13
UniProt Protein Name:C-X-C motif chemokine 13
UniProt Synonym Protein Names:B lymphocyte chemoattractant; CXC chemokine BLC; Small-inducible cytokine B13
Protein Family:Beta carbonic anhydrase
UniProt Gene Name:Cxcl13��
UniProt Entry Name:CXL13_MOUSE
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Additional Information

Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
O55038
Research Area:
Chemokines
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