Mouse Apoptosis Array (38 targets) (SARB0073)

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SKU:
SARB0073
Product Type:
Protein Array
Analytes:
bad
Analytes:
bax
Analytes:
bcl-2
Analytes:
bcl-w
Analytes:
BID
Analytes:
BIM
Analytes:
Caspase-3
Analytes:
Caspase-8
Analytes:
CD40 (TNFRSF5)
Analytes:
CD40 Ligand (TNFSF5)
Analytes:
cIAP-2
Analytes:
Cytochrome C
Analytes:
DR6 (TNFRSF21)
Analytes:
Fas (TNFRSF6/Apo-1)
Analytes:
Fas Ligand (TNFSF6)
Analytes:
HSP27
Analytes:
HSP60
Analytes:
HSP70
Analytes:
HTRA2
Analytes:
IGFBP-1
Analytes:
IGFBP-2
Analytes:
IGFBP-3
Analytes:
IGFBP-4
Analytes:
IGFBP-5
Analytes:
IGFBP-6
Analytes:
IGF-1
Analytes:
IGF-2
Analytes:
p21
Analytes:
p27/Kip1 (CDKN1B)
Analytes:
p53
Analytes:
SMAC
Analytes:
Survivin (BIRC5)
Analytes:
TNF RI (TNFRSF1A)
Analytes:
TNF RII (TNFRSF1B)
Analytes:
TNF alpha
Analytes:
TNF beta (TNFSF1B)
Analytes:
TRAIL R2 (TNFRSF10B/DR5)
Analytes:
XIAP
Reactivity:
Mouse
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0073
Size:2, 4, or 8
Species Detected:Mouse
Number of Targets Detected:38
Gene Symbols:BAD, BAX, BCL2, BCL2L11, BCL2L2, BID, BIRC3, BIRC5, CASP3, CASP8|CD40, CD40LG, CDKN1A, DIABLO, FAS, FASLG, HSPA4, HSPB1, HSPD1, HTRA2, IGF1, IGF2, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP5, IGFBP6, LTA, PER27, TNF, TNFRSF10B, TNFRSF1A, TNFRSF1B, TNFRSF21, TP53, XIAP
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:Akt Signaling / IGF Signaling / MAPK Signaling / NFkB Signaling / p53 Signaling / PI3K-AKT Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
bad
  • Gene Symbol: Bad Bbc6
  • Gene ID: 12015
  • Accession #: Q61337
bax
  • Gene Symbol: Bax
  • Gene ID: 12028
  • Accession #: Q07813
bcl-2
  • Gene Symbol: Bcl2 Bcl-2
  • Gene ID: 12043
  • Accession #: P10417
bcl-w
  • Gene Symbol: Bcl2l2 Bclw Kiaa0271
  • Gene ID: 12050
  • Accession #: P70345
BID
  • Gene Symbol: Bid
  • Gene ID: 12122
  • Accession #: P70444
BIM
  • Gene Symbol: Bcl2l11 Bim
  • Gene ID: 12125
  • Accession #: O54918
Caspase-3
  • Gene Symbol: Casp3 Cpp32
  • Gene ID: 12367
  • Accession #: P70677
Caspase-8
  • Gene Symbol: Casp8
  • Gene ID: 12370
  • Accession #: O89110
CD40 (TNFRSF5)
  • Gene Symbol: Cd40 Tnfrsf5
  • Gene ID: 21939
  • Accession #: P27512
CD40 Ligand (TNFSF5)
  • Gene Symbol: Cd40lg Cd40l Tnfsf5
  • Gene ID: 21947
  • Accession #: P27548
cIAP-2
  • Gene Symbol: Birc3 Birc2 Iap1
  • Gene ID: 11796
  • Accession #: O08863
Cytochrome C
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #: N/A
DR6 (TNFRSF21)
  • Gene Symbol: Tnfrsf21 Dr6
  • Gene ID: 94185
  • Accession #: Q9EPU5
Fas (TNFRSF6/Apo-1)
  • Gene Symbol: Fas Apt1 Tnfrsf6
  • Gene ID: 14102
  • Accession #: P25446
Fas Ligand (TNFSF6)
  • Gene Symbol: Faslg Apt1lg1 Cd95l Fasl gld Tnfsf6
  • Gene ID: 14103
  • Accession #: P41047
HSP27
  • Gene Symbol: Hspb1 Hsp25 Hsp27
  • Gene ID: 15507
  • Accession #: P14602
HSP60
  • Gene Symbol: Hspd1 Hsp60
  • Gene ID: 15510
  • Accession #: P63038
HSP70
  • Gene Symbol: Hspa4 mCG_113604
  • Gene ID: 15525
  • Accession #: Q3U2G2
HTRA2
  • Gene Symbol: Htra2 Omi Prss25
  • Gene ID: 64704
  • Accession #: Q9JIY5
IGFBP-1
  • Gene Symbol: Igfbp1 Igfbp-1
  • Gene ID: 16006
  • Accession #: P47876
IGFBP-2
  • Gene Symbol: Igfbp2 Igfbp-2
  • Gene ID: 16008
  • Accession #: P47877
IGFBP-3
  • Gene Symbol: Igfbp3 Igfbp-3
  • Gene ID: 16009
  • Accession #: P47878
IGFBP-4
  • Gene Symbol: Igfbp4 Igfbp-4
  • Gene ID: 16010
  • Accession #: P47879
IGFBP-5
  • Gene Symbol: Igfbp5 Igfbp-5
  • Gene ID: 16011
  • Accession #: Q07079
IGFBP-6
  • Gene Symbol: Igfbp6 Igfbp-6
  • Gene ID: 16012
  • Accession #: P47880
IGF-1
  • Gene Symbol: Igf1 Igf-1
  • Gene ID: 16000
  • Accession #: P05017
IGF-2
  • Gene Symbol: Igf2 Igf-2
  • Gene ID: 16002
  • Accession #: P09535
p21
  • Gene Symbol: Cdkn1a Cip1 Waf1
  • Gene ID: 12575
  • Accession #: P39689
p27/Kip1 (CDKN1B)
  • Gene Symbol: PER27 P27 At3g01190 T4P13.12
  • Gene ID: 821314
  • Accession #: Q43735
p53
  • Gene Symbol: Tp53 P53 Trp53
  • Gene ID: 22059
  • Accession #: P02340
SMAC
  • Gene Symbol: Diablo Smac
  • Gene ID: 66593
  • Accession #: Q9JIQ3
Survivin (BIRC5)
  • Gene Symbol: Birc5 Api4 Iap4
  • Gene ID: 11799
  • Accession #: O70201
TNF RI (TNFRSF1A)
  • Gene Symbol: Tnfrsf1a Tnfr-1 Tnfr1
  • Gene ID: 21937
  • Accession #: P25118
TNF RII (TNFRSF1B)
  • Gene Symbol: Tnfrsf1b Tnfr-2 Tnfr2
  • Gene ID: 21938
  • Accession #: P25119
TNF alpha
  • Gene Symbol: Tnf Tnfa Tnfsf2
  • Gene ID: 21926
  • Accession #: P06804
TNF beta (TNFSF1B)
  • Gene Symbol: Lta Tnfb Tnfsf1
  • Gene ID: 16992
  • Accession #: P09225
TRAIL R2 (TNFRSF10B/DR5)
  • Gene Symbol: Tnfrsf10b Dr5 Killer
  • Gene ID: Tnfrsf10b Dr5 Killer
  • Accession #: Q9QZM4
XIAP
  • Gene Symbol: Xiap Aipa Api3 Birc4 Miha
  • Gene ID: 11798
  • Accession #: Q60989
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Mouse Apoptosis Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • Protease Inhibitor Cocktail
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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