Mouse Cell Biology ELISA Kits 2

Mouse Apolipoprotein E / APOE ELISA Kit

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SKU:
MOFI00648
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P08226
Sensitivity:
0.938ng/ml
Range:
1.563-100ng/ml
ELISA Type:
Sandwich
Synonyms:
APO-E, Apolipoprotein E, Apo-E, MGC1571, apolipoprotein E3
Reactivity:
Mouse
Research Area:
Cell Biology

Description

Mouse Apolipoprotein E / APOE ELISA Kit

APOE is a vital protein involved in lipid metabolism and transportation, playing a crucial role in maintaining lipid homeostasis and clearance. This high-quality ELISA kit provides a reliable and sensitive method to accurately quantify APOE levels in mouse serum, plasma, and other biological samples, enabling researchers in understanding the role of APOE in various physiological and pathological processes, such as cardiovascular diseases, neurodegeneration, and lipid-related disorders.

system_update_alt Datasheet system_update_alt MSDS

Key Features

Save Time Pre-coated 96 well plate
Quick Start Kit includes all necessary reagents
Publication Ready Reproducible and reliable results

Overview

Product Name:

Mouse Apolipoprotein E / APOE ELISA Kit

Product Code:

MOFI00648

Size:

96 Assays

Alias:

APO-E, Apolipoprotein E, Apo-E, MGC1571, apolipoprotein E3

Detection Method:

Sandwich ELISA

Application:

This immunoassay kit allows for the in vitro quantitative determination of Mouse APO-E concentrations in serum plasma and other biological fluids.

Sensitivity:

0.938ng/ml

Range:

1.563-100ng/ml

Storage:

4°C for 6 months

Note:

For Research Use Only

Additional Information

Recovery

Matrices listed below were spiked with certain level of Mouse APO-E and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse APO-E in samples.

Matrix

Recovery Range (%)

Average (%)

serum (n=5)

86-99

92

EDTA plasma (n=5)

85-104

94

UFH plasma (n=5)

85-99

91

Linearity:

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse APO-E and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

Serum (n=5)

95-104%

88-102%

87-104%

EDTA plasma (n=5)

82-101%

82-100%

82-101%

UFH Plasma (n=5)

80-90%

84-100%

80-95%

CV(%)

Intra Assay <8

Inter Assay <10

Kit Components

Component Quantity Storage

ELISA Microplate (Dismountable)

8x12 strips

4°C for 6 months

Lyophilized Standard

2

4°C/ -20°C

Sample/Standard Dlution Buffer

20ml

4°C

Biotin-labeled Antibody (Concentrated)

120ul

4°C (Protection from light)

Antibody Dilution Buffer

10ml

4°C

HRP-Streptavidin Conjugate (SABC)

120ul

4°C (Protect from light)

SABC Dilution Buffer

10ml

4°C

TMB Substrate

10ml

4°C (Protection from light)

Stop Solution

10ml

4°C

Wash Buffer (25X)

30ml

4°C

Plate Sealer

5

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