Mouse ANXA1 Recombinant Protein (RPPB2748)

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SKU:
RPPB2748
Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P10107

Description

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Product Name:Mouse ANXA1 Recombinant Protein
Product Code:RPPB2748
Size:20µg
Species:Mouse
Target:ANXA1
Synonyms:Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2,Chromobindin-9, Lipocortin I, PhospholipaseA2 inhibitory protein, p35.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:ANXA1 protein solution (1mg/ml) containing Phosphatebuffered saline (pH7.4), 20% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAMVSEF LKQARFLENQ EQEYVQAVKS YKGGPGSAVS PYPSFNVSSDVAALHKAIMV KGVDEATIID ILTKRTNAQR QQIKAAYLQE NGKPLDEVLR KALTGHLEEV VLAMLKTPAQFDADELRGAM KGLGTDEDTL IEILTTRSNE QIREINRVYR EELKRDLAKD ITSDTSGDFR KALLALAKGDRCQDLSVNQD LADTDARALY EAGERRKGTD VNVFTTILTS RSFPHLRRVF QNYGKYSQHD MNKALDLELKGDIEKCLTTI VKCATSTPAF FAEKLYEAMK GAGTRHKALI RIMVSRSEID MNEIKVFYQK KYGISLCQAILDETKGDYEK ILVALCGGN

ANXA1 is part of the family of Ca(2+)-dependent phospholipid binding proteins which have a Mw between 35kDa-40kDa and are situated on the cytosolic face of the plasma membrane. ANXA1 protein has a Mw of 40kDa, with phospholipase A2 inhibitory activity to bind from two to four calcium ions with high affinity. Since phospholipase A2 is necessary for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, ANXA1 might have potential anti-inflammatory activity. ANXA1 promotes membrane fusion and iplays a role in exocytosis. The recognition of ANXA1 protein by immunocytochemical leads a simple, highly sensitive and specific assay for diagnosis of hairy cell leukemia.

ANXA1 Mouse Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 369 amino acids (1-346 a.a) andhaving a molecular mass of 41.1kDa.ANXA1 is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.

UniProt Protein Function:ANXA1: a calcium/phospholipid-binding protein with which promotes membrane fusion and is involved in endocytosis. Has anti-inflammatory properties and inhibits phospholipase A2 activity. Accumulates on internalized vesicles after EGF-stimulated endocytosis, suggesting that it may be required for a late stage in inward vesiculation. Phosphorylated by PKC, EGFR and Chak1. Phosphorylation results in loss of the inhibitory activity. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.Protein type: Lipid-binding; Calcium-bindingChromosomal Location of Human Ortholog: 9q21.13Cellular Component: extracellular space; mast cell granule; cell surface; focal adhesion; protein complex; basolateral plasma membrane; extracellular region; cornified envelope; cilium; mitochondrial membrane; cytoplasm; plasma membrane; nucleus; vesicle; sarcolemma; endosomeMolecular Function: protein binding, bridging; protein binding; protein homodimerization activity; single-stranded RNA binding; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; double-stranded DNA-dependent ATPase activity; phospholipid binding; calcium ion binding; structural molecule activity; calcium-dependent protein binding; helicase activity; single-stranded DNA binding; receptor bindingBiological Process: response to drug; prostate gland development; response to peptide hormone stimulus; positive regulation of neutrophil apoptosis; gliogenesis; positive regulation of vesicle fusion; alpha-beta T cell differentiation; arachidonic acid secretion; neutrophil homeostasis; signal transduction; endocrine pancreas development; response to estradiol stimulus; DNA duplex unwinding; regulation of cell proliferation; keratinocyte differentiation; cell surface receptor linked signal transduction; insulin secretion; DNA strand renaturation; peptide cross-linking; inflammatory response; cell motility; response to X-ray; positive regulation of prostaglandin biosynthetic process; negative regulation of apoptosis
UniProt Protein Details:
NCBI Summary:This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014]
UniProt Code:P10107
NCBI GenInfo Identifier:4502101
NCBI Gene ID:301
NCBI Accession:NP_000691.1
UniProt Secondary Accession:P10107
UniProt Related Accession:P10107,P04083
Molecular Weight:Calculated MW: 38714
NCBI Full Name:annexin A1
NCBI Synonym Full Names:annexin A1
NCBI Official Symbol:ANXA1��
NCBI Official Synonym Symbols:ANX1; LPC1��
NCBI Protein Information:annexin A1
UniProt Protein Name:Annexin A1
UniProt Synonym Protein Names:Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35
Protein Family:Annexin
UniProt Gene Name:ANXA1��
UniProt Entry Name:ANXA1_HUMAN
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